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Regulation mechanism of nsulin-like growth factor-binding proteins producion in osteoarthritis.

Research Project

Project/Area Number 09671503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNagasaki University

Principal Investigator

MATSUMOTO Tomoko  Nagasaki University, School of Medicine Hospital, Assistant, 医学部附属病院, 助手 (30239107)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Shunichi  Nagasaki University, School of Medicine, Professor, 医学部, 教授 (30200679)
ENOMOTO Hiroshi  Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (90284679)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsOsteoarthritis / IGF-bimding protein / Isulin-like growth factor-1
Research Abstract

Thc destruction of cartilage tissue causes functional disability in osteoarthritis. The repair of cartilage tissue are supposed to be disturbed in this disease. Insulin-like growth factor (IGF) is an anabolic factor for chondrocytes and is regulated by binding proteins (IGPBPs). We assumed that the effect of IGF-I on chondrocytes are disturbed in osteoarthritis. So we tried to study the IGFBPs produced by human chondrocytes, which may regulate IGE action. Employing Western-ligand blotting (WLB) and immunoprecipitation, immortalized humah chondrocytes were found to secrete IGFBP-2, -3, -4 and -5. Treatment of cells with IGF-I resulted in a dose-dependent increase of IGFBP-5. The IGFBPs associated with cell membrane were examined by affinity cross-linking method, showing the IGFBP-3 and -5 as well as type 1 LGF and type 2 [GE receptor. To clarify the changes of IGFBPs in osteoarthritis, interleukin-1b is added to the culture medium. Unfortunately, there was no difference between them, with /without interleukin-1. We should further study the regulation mechanism of IGEBP production in osteoarthritis by using human cartilage tissue obtained by operated sample.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] MITO: "microarchitecture of human iliac bone using microcomputed tomography in patients with hip arthrosis with or without vertebral fracture." Bone. 23. 163-169 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Matsumoto: "Decreased levels of IGF binding protein-3 in serum from children with Perthes' disease." Acta Orthopedica Scandinavica. 69. 125-128 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Matsumoto: "Increased levels of IGF-I and IGFBP-3 in synovial fluids of patients with rheumatoid arthritis." Endocrine Journal. 45. S141-144 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Adashi EY: "The mouse intraovarian insulin-like growth factor 1 system: departure from the rat paradigm." Endocrinology. 138. 3881-3890 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] D Choi: "Activin-attenuated expression of granulosa cell-derived insulin-like growthfactor binding protein 4 and 5:a putative antiatretic effect." Biology of Reproduction. 56. 508-515 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] MITO: "Analysis of trabecular microarchitecture of human iliac bone using microcomputed tomography in patients with hip arthrosis with or without vertebral fracture." Bone. 23. 163-169 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] MATSUMOTO T: "Increased levels of IGF-I and IGFBP-3 in synovial fluids of patients with rheumatoid arthritis." Endocrine Journal. 45. s141-144 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Matsumoto: "Decreased levels of IGF binding protein-3 in serum from children with Perthes' disease." Acta Orthop Scandinavica. 69. 125-128 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Adashi EY.: "The mouse intraovarian insulin-like growth factor 1 System : departure from the rat paradigm." Endocrinology. 138. 3381-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Choi D: "Activin-attenuated expression of granulosa cell-derived insulin-like growthfactor binding protein 4 and 5 : a putative antiatretic effect." Biology of Reproduction. 56 (2). 508-15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Choi D,: "Activin-attenuated expression of granulosa cell-derived insulin-like growthfactor binding protein 4 and 5:a putative antiatretic effect." Biology of Reproduction. 56. 508-515 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Adashi EY: "The mouse intraovarian insulin-like growth factor 1 system:departure from the rat paradigm." Endocrinology. 138. 3881-3890 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto T,: "Increased levels of IGF-I and IGFBP-3 in synoyial fluids of patients with rheumatoid arthritis." Endocrine Journal. 45. S141-S144 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto T,: "Decreased levels of IGF binding protein-3 in serum from childen with Perthes' disease." Acta Orthopedica Scandinavica. 69. 125-128 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto T: "Transcriptional and post-translational regulation of insulin-like growth factors-binding proteins (IGFBP)-5 in rat articular chondrocytes." J Endocrinology. 148. 355-369 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto T: "Identification and characterization of insulin-like growth factors (IGFs),IGFBPs and IGFBP proteases in human synovial fluid." Journal of Clinical Endocrinology and Metabolism.81. 150-155 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto T: "Characterization of insulin-like growth factor binding protein-5 and protease produced by rat articular chondrocyte and a neuroblastoma cell line." Growth Regulation. 6. 185-190 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto T: "The mechanism of induction of insulin-like growth factor binding protein (IGFBP)-5 by IGFs in rat articular chondrocytes." Endocrine Journal. 43. S93-S95 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto T: "Increased levels of IGFT-I and IGFBP-3 in synovial fluids of patients with rheumatoid arthritis" Endocrine Journal. 45(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto T: "Effects of interleukin-1β on insulin-like growth factor-I autocrine/paracrine axis in cultured rat articular chondrocytes." Annals of Rheumatic Diseases. 53. 128-133 (1994)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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