| Project/Area Number |
09671519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
FUJIMURA Yoshikazu Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (30201750)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Masahiko Keio University, School of Medicine, Instructor, 医学部, 助手 (40220925)
小川 祐人 大阪大学, 医学部, 助手 (30265855)
小粥 博樹 慶應義塾大学, 医学部, 助手 (70255488)
中村 雅也 慶應義塾大学, 医学部, 助手 (30217898)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | spinal cord injury / motoneuron / nerve growth factor / p-75 / glial cell line-derived neurotrophic factor / choline acetyltransferase / コリンアセチル基転移酵素 / 神経栄養因子受容体 / 神経成長因子 / 神経成長因子受容体 / Glial cell line-derived neurotrophic factor |
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| Research Abstract |
We investigated the influences of neurotrophic factor on injured motoneurons in acute spinal cord injury models. Our study consists of the following two experiments
Experiment 1.25 Wister rats were used in this experiment. An incomplete spinal cord injury model was created by placing a 20-g weight on the dura mater at the C6 level for 5 min (n=20) .The cervical spinal cord was remove at post-injury day 3,7,14,28, and frozen sections were stained for p-75 .The number of p-75 positive motoneurons were counted. (Results) There were no p-75 positive motoneurons in the sham group(n=5). In the injury group, p-75 positive motoneurons were recognized at post-injury day 3, highly increased at day 7, and hardly observed by at day 14, 28.
Experiment. 2; 20 incomplete spinal cord injury models were created in the same manner. In 10 glial cell line-derived neurotrophic factor(GDNF) was injected into the subarachnoid space immediately after the injury, ,and phosphate buffer saline (PBS) was injected n the other 10 animals. Motor function was evaluated at post-injury day 2 and 7. The spinal cord was removed from both groups 7 days after the injury. The sections were immunofluorescence stained for choline acetyltransferase (ChAT). ChAT fluorescence was measured with a microphotometry system. (Results) Motor function evaluation: Both the GDNF group and the PBS group were able to maintain their trunk on the inclined plane up to a mean angle of 82゜before the injury. On post-injury day 2 the angle at which they, were able to maintain their trunk had decreased to 55゜and 52゜, respectively, and on day 7, to 68゜and 63゜ respectively. The deference on post-injury day 2 was not significant, but a significant deference was observed by day 7. Immunohistochemical findings: The fluorescence values in both injured groups were lower than in the uninjured group, but the reduction in fluorescence that occurred in the GDNF group was a significantly prevented.
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