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Muscle fiber necrosis and oxidative stress in disuse muscular atrophy

Research Project

Project/Area Number 09671531
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

HACHISUKA Kenji  UOEH, School of Med.Prof., 医学部, 教授 (00129602)

Co-Investigator(Kenkyū-buntansha) NANRI Hiroki  UOEH, INSTITUTE OF INDUSTRIAL ECOLOGICAL SCIENCES.Associate Prof., 産業生態科学研究所, 助教授 (80150415)
OGATA Hajime  UOEH, School of Med.Emeritus Prof., 医学部, 名誉教授 (70122843)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordshindlimb suspension / muscle atrophy / necrosis / SOD / 廃用 / 筋萎縮 / desmin / plectin / 廃用性筋萎縮 / 壊死 / トコフェロール / 酸化的ストレス / ビタミンE / 後肢懸垂
Research Abstract

The purpose of our study was to determine whether hindlimb suspension induce necrosis in the atrophied muscles and whether oxidative stress play any role in the process. Light and electron microscopic observation of hindlimb muscles of Wistar rats (8 weeks of age, male), which were unloaded for two weeks, and control rats were performed. The fiber diameters of the rectus femoris, vastus medialis, vastus lateralis, intermedius, gastrocnemius, soleus muscles of the suspended rats were smaller than those of the controls, especially type 1 fiber in the soleus muscle and type 2B fiber in the vastus lateralis and medialis muscles. Necrotic fibers were found in deep portions of the vastus lateralis, vastus medialis, lateral and medial heads of gastrocnemius, and soleus muscles of the suspended rats, and the following were the rate of necrotic fibers (number/10 rats) : 1.4, 1.3, 1.7, 1.4, 0.3, and 1.9, respectively. Reticular structures stained with monoclonal antibodies of desmin and plectin were irregular after hindlimb suspension, and irregularity of myofibrillar arragement and Z-bands was also found by electron microscopy. A few necrotic fibers in the deep portion of the hindlimb muscles had a positive reaction to the monoclonal antibody to superoxide dismutase. Alfa-tocopherol content of the soleus muscle was approximately 40% as much as that of the rectus femoris muscle. In conclusion, hindlimb suspension induces muscle fiber necrosis in the deep portion of the muscles, which were rich in oxidative fibers, and disuse muscle atrophy is not only decrease in muscle fiber diameter, but also complicated dynamic process of the muscle. Although oxidative stress may concern the process of necrosis after hindlimb suspension, α-tocopherol content did not reveal all mechanism of muscle fiber necrosis.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 蜂須賀研二,奈良聡一郎,緒方甫: "ラット下肢筋内トコフェロールの定量."日本リハビリテーション医学会. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 奈良聡一郎,堂園浩一朗,福田冬季子,伊藤政孝,杉江秀夫,蜂須賀研二,緒方甫: "高齢ラットの廃用性筋萎縮に関する組織学的,生化学的検討."日本リハビリテーション医学会. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 奈良聡一郎,梅津祐一,蜂須賀研二,緒方甫: "高齢ラットの廃用性筋萎縮に関する組織化学的検討."日本リハビリテーション医学会. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 奈良聡一郎,古川博,蜂須賀研二: "廃用性筋萎縮における形態学的,免疫組織学的検討."日本臨床電子顕微鏡学会. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hachisuka K, Nara S, Ogata H: "Tocopherol content of rat lindlimb muscles."Japanese Association of Rehabilitation Medicine. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nara S, Dozono K, Fukuda T, Itoh M, Sugie H, Hachisuka K, Ogata H: "Histological and chemical study on disuse muscle atrophy of aged rats."Japanese Association of Rehabilitation Medicine. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nara S, Umezu Y, Hachisuka K, Ogata H: "Histochemical study on disuse muscle atrophy of aged rats."Japanese Association of Rehabilitation Medicine. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nara S, Furukawa H, Hachisuka K: "Morphological and immunohistological study on disuse muscle atrophy."The Clinical Electron Microscopy Society of Japan. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary

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Published: 1997-04-01   Modified: 2016-04-21  

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