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Mechanisms of anesthetics on in situ normotensive vs hypertensive rat mesenteric vascular smooth muscle transmembrane potentials

Research Project

Project/Area Number 09671549
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionToyama Medical and Pharmaceutical University

Principal Investigator

YAMAZAKI Mitsuaki  Toyama Medical and Pharmaceutical University, University Hospital, Intensive Care Unit, Associate Professor, 附属病院, 講師 (70158145)

Co-Investigator(Kenkyū-buntansha) SHIBUYA Nobuko  Toyama Medical and Pharmaceutical University, University Hospital, Anesthesiolog, 附属病院, 講師 (40178926)
HATAKEYAMA Noboru  Toyama Medical and Pharmaceutical University, University Hospital, Operating The, 附属病院, 助手 (70251907)
ITO Yusuke  Toyama Medical and Pharmaceutical University, Faculty of Medicine, Anesthesiolog, 医学部, 教授 (70018307)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsvascular smooth muscle / transmembrane potential / isoflurane / sevoflurane / propofol / WKY rat / SHR rat / potassium channel / hypertension / normotension / vascular smooth muscle / anesthetics / membrane potential / peripheral vascular tone / splanchnic resistance / splanchnic capacitance
Research Abstract

Since tight linear coupling exists between vascular smooth muscle (VSM) transmembrane potential (Em) and active VSM tone, such hyperpolarization reflects a vasorelaxation.
In normotensive and hypertensive rats under basal anesthesia, we externalized a loop of jejunum containing mesenteric arteries and veins through a midline laparotomy and superfused them with physiological salt solution. Ems were recorded from in situ mesenteric resistance (arteries) and capacitance (veins) vessels with or without denervation (by 6- hydroxydopamine) using glass microelectrode. In normotensive rats, inhaled anesthetics (isoflurane, sevoflurane) and ketamine hyperpolarized in both mesenteric arteries and veins. Inhaled anesthetics attenuated both sympathetic neural and nonneural regulation of VSM transmembrane potentials ( and tone). Inhaled anesthetics hyperpolarized in hypertensive rats more negative than in normotensive rats.
Selective inhibitors of each of four types of potassium channels known to exist in VSM were administered in superfusate of the vessel preparations (in normotensive rats) to assess their effects on isoflurane-mediated hyperpolarization. Isoflurane-mediated hyperpolarization of VSM can be attributed in part to an enhanced (or maintained) opening of calcium-activated and adenosine triphosphate sensitive potassium channels but not voltage-dependent or inward rectifier potassium channels.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Yamazaki M, et al.: "Effects of volatile anesthetic agents on in situ vascular smooth muscle transmembrane potential in resistance- and capacitance-regulating blood vessels." Anesthesiology. 88・4. 1085-1095 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 永川保 他: "ケタミンによるin situラット腸管膜動・静脈平滑筋の膜電位の変化." 北陸麻酔学雑誌. 32・1. 1-6 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kokita N, et al.: "Potassium channel-mediated hyperpolarization of mesenteric vascular smooth muscle by isoflurane." Anesthesiology. 90・3 印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamazaki M,Stekiel TA,Boshjak ZJ,Kampine JP,Stekiel WJ.: "Effects of volatile anesthetic agents on in situ vascular smooth muscle transmenbrane potential in resistance-and capacitane-regulating blood vessels." Anesthesiology. Vol.88, No.4. 1085-1095 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagakawa T,Yamazaki M,Hatakeyama N,Shibuya N,Ito Y.: "Effects of ketamine on in situ rat mesenteric vascular smooth muscle transmembrane potentials." Hokuriku J.Anesth.Vol.32, No.1. 1-6 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kokita N,Stekiel TA,Yamazaki M,Bosnjak ZJ,Kampine JP,Stekiel WJ.: "Potassium channel-mediated hyperpolarization of mesenteric vascular smooth muscle by isoflurane." Anesthesiology. Vol.90, No.3 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamazaki M,et al.: "Effects of volatile anesthetic agents on in situ vascular smooth muscle transmembrane potential in resistance- and capacitance-regulating blood vessels." Anesthesiology. 88・4. 1085-1095 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 永川 保 他: "ケタミンによるin situラット腸管膜動・静脈平滑筋の膜電位の変化." 北陸麻酔学雑誌. 32・1. 1-6 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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