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Elucidation of Intracellular Signal Transduction Pathway Involved in the Processing of Nociceptive Information -- Focusing on the Role of Neurotrophic Factor --

Research Project

Project/Area Number 09671581
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

IBUKI Takae  Kyoto Prefectural University of Medicine, Department of Anesthesiology, Assistant Professor, 医学部, 講師 (90232587)

Co-Investigator(Kenkyū-buntansha) HARADA Masato  Kyoto Prefectural University of Medicine, Department of Anesthesiology, Instruct, 医学部, 助手 (20260797)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordspain / signal transduction / allodynia / GABA / glycine / hyperalgesia / spinal dorsal horn / neuropathy / 疼覚 / 情報伝達系
Research Abstract

1. In the chronic constriction nerve injury model, the time course of changes in hyperalgesia and allodynia were quantitatively assessed and the high correlation between this time course and that of morphological changes in GABAergic inhibitory interneurons in the spinal dorsal horn was proved. The number and immunoreactivity of these GABAergic interneurons begin to decrease ipsiaterally at 3 days following nerve injury and no immunoreactivity could be observed bilaterally during the 3 weeks following injury. In the contralateral side, GABAergic immunoreactivity recovered approximately to the presurgical level at about 7 weeks after injury ; on the other hand in the ipsilateral side the recovery was incomplete even at 7 weeks following surgery. The time course of changes in the degenerated neurons in the spinal dorsal horn (Dark neurons) was similar to that of these changes.
2. In the spinal nerve injury model, the time course of changes in GABAergic and glycinergic inhibitory interneurons in the superficial dorsal horn of the spinal cord were observed. A slight decrease in GABA immunoreactivity was observed bilaterally 3 days following surgery. Bilateral decrease in GABA immunoreactivity was accelerated at I week and continued for at least 3 weeks after surgery, when GABAergic immunoreactive components began to recover. Glycinergic fibers and terminals diminished 1 week ater nerve injury and the time course of the changes followed a similar course.
3. One of the neurotrophic factors, saposin was proved to be effective in the alleviation of some pain behavior in neuropathic pain models.
4. In order to evaluate the effect of various neurotrophic factors, adrenal medurally transplantation was performed using the CCI model. The therapeutic effect of adrenal medullary transplants on the vulnerable inhibitory interneurons in the spinal dorsal horn was proved and acceralated recovery of behavioral hyperalgesia and allodynia was evident.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] T.Ibuki,A.T.Hama,X.-T.Wang,G.D.Pappas and J.Sagen: "Loss of GABA-Immunoreactivity in the spinal dorsal horn of rats with peripheral nerve injury and promotion of recovery by adrenal medullary grafts" NeuroScience. 76(3). 845-858 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki,S.A.Dunbar and T.L.Yaksh: "Effect of Transient Naloxone Antagonism on Tolerance Development in Rats Receiving Continuous Spinal Morphine Infusion" Pain. 70. 125-132 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki,A.T.Hama,G.D.Pappas and J.Sagen: "Spinal GABA ergic neurons involved in the process of neuropathy and therapeutic effects of adrenal medullary transplantation" Proc.Soc Neurosci. 2. 1533- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki,M.Marsala and T.L Yaksh: "Spinal amino acid release and antinociceptive tolerance in spinal morphine tolerant rats : Effect of repeated daily withdrawal" Submission.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Marsala,T.Ibuki,K.H Jhamandas and T.L.Yoksh: "Spinal amino acid and prostaglandin E2 release after withdrawal in rats infused with spinal morphine" Submission.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang,X.T.,Unnersfall,J.,Ibuki,T.,Wang,H.,Pappas,G.D.and Sagen J.: "Comporisons between tyrosine hyroxylase and preproenhephalin mRNA in rat adrenal nedullary explants in vitro and in CNS transplants" Submission.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki and Y.Tanaka: "Changes of GABA ergic and glycinergic inhibitory heuronal system fellowing nerve injury" Proc Soc Neurosci 2. 1392- (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.L.Yaksh,M.Marsala and T.Ibuki: "Release of glutamate during opioid exposure" Pain Forum in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Matsumoto,S.Hayashi T.Ibuki,M.Tanaka,Y.Tanaka: "Morphological and functional roles of macropharges and postganglionic sympathetic fibers in dorsal root ganglions of a rat neuropathic pain model" submission.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki,M.Morsala and T.L.Yaksn: "General anesthesia inhibits the release of spinal amino acids and prostaglandin E2 and precipitated withdrawal in inorphine tolerant rats." Submission.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki, A.T.Hama, X-T Wang, G.D.Pappas and J.Sagen: "Loss of GABA-Immunoreactivity in the spinal dorsal born of rats with peripheral nerve injury and promotion of recovery by adrenal medullary grafts" Neuroscience. 76 (3). 845-858 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki, S.A.Dunbar and T.L.Yaksh: "Effect of Transient Naloxone Antogonism on Tolerance Development in Rats Receiving Continuous Spinal Morphine Infusion" Pain. 70. 125-132 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takae Ibuki, A.T.Hama, G.D.Pappas and J.Sagen: "Spinal GABAergic neurons involved in the process of neuropathy and therapeutic effects of adrenal medullary transplantation" Proc Soc Neurosci. 2. 1533 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki, M.Marsala and T.L.Yaksh: "Spinal amino acid release and antinociceptive tolerance in spinal morphine tolerant rats : Effect of repeated daily withdrawal" submission.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Marsala, T.Ibuki, K.H.Jhamandas and T.L.Yaksh: "Spinal amino acid and prostaglandin E2 release after withdrawal in rats infused with spinal morphine" submission.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang, X-T., Unnerstall, J., Ibuki, T., Wang, H., Pappas, G.D.and Sagen J.: "Comparisons between tyrosine hydroxylase and preproenkephalin mRNA in rat adrenal medullary explants in vitro and in CNS transplants"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki and Y.Tanaka: "Changes of GABAergic and glycinergic inhibitory neuronal system following nerve injury" Proc Soc Neurosci. 2. 1392 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.L.Yaksh, M.Marsala and T.Ibuki: "Release of glutamate during opioid exposure" Pain Forum. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Matsumoto, S.Hayashi, T.Ibuki, M.Tanaka Y.Tanaka and Y.Ibata: "Morphological and functional roles of macropharges and postganglionic sympathetic fibers in dorsal root ganglions of a rat neuropathic pain model" submission.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki, M.Marsala and T.L.Yaksh: "General anesthesia inhibits the release of spinal amino acids and prostaglandin E2 and precipitated withdrawal in morphine tolerant rats" submission.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ibuki, A.T.Hama, X-T Wang G.D.Pappas and J.Sagen: "Loss of GABA-Immuncredctivity in the Spinal dorsal horn of rats with peripheral herve injury and promotion of recovery by adrenal medullary gkafts." Nearoscirence. 76(3). 845-858 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T. Ibuki, S.A.Dunbar and T.L.Yaksh: "Effect of Transient Naloxone Antogonism on Tolerance Development in Rats Receiving Continuous Spinal Morphine Infusion" Pain. 125-132 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Ibuki, A.T.Hama, G.D.Pappas and J.Sagen: "Spinal GABA ergic neurons involved in the process of rcuropathy and therapentic effects of adrenal medullary transplantation" Proc Soc Neurosc. 1533 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Ibuki, M.Marsala and T.L.Yaksh: "Spinal amino acid release and anfinociceptive folerance in Spinal norphine folerant rats : Effect of repeated daily with drawal" submission.

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Marsala, T.Ibuki, K.H.Thamandas and T.L.Yaksh: "Spinal amino acid and prostaglandin E2 release affer with drawal in rats infused with spinal morphine" submission.

    • Related Report
      1998 Annual Research Report
  • [Publications] Wang,X.-T., Unnerstall, J., Ibuki, T.Wang, H., Pappas G.D and Saspen,J.: "Can porisons between tyrosine hydroxylase and preproenkephalin mRNA in rat adrenal medullary explants in vitroand in CNS fransplants" Submission.

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Ibuki and Y.Tanaka: "Changes of GABA ergic and glycinergic inhibitory neuronal system following nerve injury" Proc Sec Neurosci 2. 1392 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.L.Yaksh, M.Marsala and T.Ibuki: "Release of glutamate during opioid exposure" Pain Forum. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Matsumoto, S.Hayashi, T.Ibuki, M.Tanaka, Y.Tanaka and Y.Tbata: "Morphological and functional rctes of macrophargos and Destganglionic sympathetic fibers in dorsal root gnglions of a rat ncuropathic pain model" Subnission.

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Ibuki, M.Marsala and T.L.Yaksn: "General ancsthesia inhibits the release of spinal amino acids and prostoglendin E_2 and precipitated withchcowal in inorphine" Submission.

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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