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Why do anesthesia and surgical procedures deteriorate host defense ability in perioperative period?

Research Project

Project/Area Number 09671587
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNippon Medical School

Principal Investigator

OGAWA Ryo  Nippon Medical School, Professor, 医学部, 教授 (20008345)

Co-Investigator(Kenkyū-buntansha) HOSHINO Ken  Nippon Medical School, Assistant, 医学部, 助手
KIM Chol  Nippon Medical School, Assistant, 医学部, 助手
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsSurgical stress / host defense inability / lypphocytepenia / apoptosis / TNF / 宿主防衛機能 / リンパ球
Research Abstract

The purpose of this study is to invetigate the mechanism by which host-defence inability has been produced in patients who underwent surgical treatment.
The findings obtained are followed ; (1)The number of lymphcytes in periperal blood decreased after surgical procedures. The compositions of lymphocytes were separated into helper t-cells (CD4) and supressort-cells (CD8) by cyteflow metry. The ratio of CD4 and CD8 (CD4/CD8) decreased significantly after upper abdominal operations.(2) The lymphocytes were separated by centrifugation and cultured in a medium. Then the nuclear chromatin was stained by an indicator pigment. Microscopic examination revealed an increased fragmentation rate of nuclear chomatin in surgical patients.(3) The lymphocytes were incubated in a medium containing fas-antibody and the ratio of labelled cells were analysed, showing an increased staining in surgical patients.(4)The periphera bloods wete taken from postoperative patients to determine hormones such as cortisol and norepinephrine, and cytokines such as IL-I and TNF.The concentration of TNF in blood correlated very well to fragmentation rate of nuclear chromatin.
Th results suggest that surgical invasion stimulate the destruction of peripheral lymphocytes by apoptotic process in postoperative perid.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 小川龍: "侵襲制御の意議と展望" 日本歯科麻酔学会雑誌. 25・3. 315-319 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TakedaS,OgawaR: "The effect of preoperative high dose methyl, prednisolone in atenuating the metabolic response after Oesphayeal resection" Enr J Surg. 163. 511-517 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小川龍: "SIRS対策としてのステロイド" アレルギー・免疫. 6・1. 105-108 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小川龍: "ICUにおける侵襲と生体反応" 集中治療. 10・9. 925-932 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小川龍: J.Anesth. 10.Supp. 787 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogawa R: "Significance and perspection of control of surgical stress" J Society of Dental Anesthesia. 25(3). 315-319 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadeda S et al: "The effects of preoperative high dose methylprednisolone in attenuating the metabolic response after oesophageal resection" Eur J Surg. 163(3). 511-517 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogawa R: "Steroid as a measure to conunteract SIRS." Allery and Immunology. 6(1). 105-108 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ogawa R: "Stress and body response in intensive care unit" Intensive Care Med. 10(9). 926-932 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小川龍: "一酸化窒素による血管内皮細胞の障害" Journal of Anesthesia. 12・Suppl. 578 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2017-10-12  

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