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ROLE OF NMDA AND AMPA/KINATE RECEPTOR CHANNELS IN THE PREVENTION OF BLADDER OVERACTIVITY AFTER CEREBRAL INFARCTION

Research Project

Project/Area Number 09671619
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKanazawa University

Principal Investigator

KOMATSU Kazuto  University Hospital, Kanazawa University Assistant Professor, 医学部・附属病院, 助手 (80291368)

Co-Investigator(Kenkyū-buntansha) KOH Eitetsu  University Hospital, Assistant Professor, 医学部・附属病院, 助手 (90283134)
YOKOYAMA Osamu  University Hospital, Assistant Professor, 医学部・附属病院, 講師 (90242552)
NAMIKI Mikio  School of Medicine, Professor, 医学部, 教授 (70155985)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsStroke / neurogeric bladder / bladder overactivity / NMDA / antisense / glutamate / stroke
Research Abstract

ROLE OF NMDA AND AMPA/KINATE RECEPTOR CHANNELS IN THE - PREVENTION OF BLADDER OVERACTIVITY AFTER CEREBRAL INFARCTION
INTRODUCTION AND OBJECTIVES : To investigate the role of glutamatergic receptors in detrusor hyperreflexia after cerebrovascular disease, we examined the effect of two different types of glutamate receptor antagonists on bladder function after focal cerebral infarction in rat model.
METHODS : Focal cerebral infarction was produced with a middle cerebral artery occlusion (MCAO) technique on the left side in female S-D rats under halothane anesthesia. Cystometric examination was performed on unanesthetized rats through a catheter implanted in the dome of the urinary bladder. Entire experiments was designed to allow for observation of acute phase (0 to 8 hours) reaction after MCAO.Two kinds of glutamate receptor antagonists were administrated intravenously before or after MCAO.
RESULTS : Bladder capacity of conscious rats was significantly reduced just after MCAO and remained comparatively small for 8 hours. Intravenous administration of N -methyl-d-aspartate (NMDA) receptor antagonist MK-801. (0.5 mg/kg) prior to MCAO prevented the reduction in bladder capacity. This effect becaome gradually observable about 4 hours after MCAO.The bladder capacity of rats given a-amino -3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)/kinate receptor antagonist NBQX (10, 30 mg/kg) prior to MCAO was not different from that of rats with vehicle administration followed by MCAO.Administration of MK-801(0.5 mg/kg) or NBQX (30 mg/kg) after MCAO did not inhibit the reduction in bladder capacity.
CONCLUSIONS : Our results suggest that glutamate may play a part in the pathogenesis of voiding dysfunction after cerebrovascular disease, and that the NMDA receptor may play a more dominant role than the AMPA/kinate receptor in evoking bladder overactivity after MCAO.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] KOMATSU,K: "An antisense to NMDA-R1 relieves decrease in bladder capacity after focal cerebral infarction in rats" Neurourology and Urodynamics. 16(5). 469-470 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA,O: "Effect of MK801 on bladder overactivity in rats with cerebral infarction" J.Urology. 159. 571-596 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA,O: "Change in blodder contractility associated with bladder over activity in rats with cerebral infarction" J.Urology. 159. 577-580 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水野 宏昭: "脳梗塞ラットの過活動型膀胱発生機序に関する薬理学的検討" 金沢大学大学院薬学研究科医療薬学専攻 医療薬剤学講座 学位論文, 41 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Komatsu, K,Ishiura, Y.Nakamura, Mita E,Mizuno H,Koh E,Yokoyama O,Namiki M,Aoki M,Morishita R and Higaki J: "An antisense to NMDA-R1 relieves decrease in bladder capacity after focal cerebral infarction in rats." Neurourol Urodyn. 16. 469-470 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokoyama, O,Ihiura, Y,Komatsu, K,Mita, E,Nakamura, Y,Kunimi, K,Morikawa, K and Namiki, M: "Effects of MK-801 on bladder overactivity in rats with cerebral infarction." J.Urol. 159. 571-576 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokoyama, O,Komatsu, K,Ishiura, Y,Nakamura, Y,Morikawa, K and Namiki, M: "Change in bladder contractility associated with bladder overactivity in rats with cerebral infarction." J.Urol. 159. 577-580 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KOMATSU, K: "An antisense to NMDA-R1 Relitves decreate in bladder capacity after focal cerebral infarction in rats." Neurourology and Urodynamics. 16(5). 469-470 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] YOKOYAMA, O: "Effect of Me801 on bladder overactivity in rats with cerebral infarction" J.Urology. 159. 571-576 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] YOKOYAMA, O: "Change in bladder contractility associated with blodder overactivity in rats with cerebral infarction" J.Urology. 159. 577-580 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizuno, H: "Role of NMDA and AMPA/KINATE receptor channels in the prevention of bladder overactivity after cerebral infarction in ratsn" J.Urology. 161. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 水野 宏昭: "脳梗塞ラットの過活動型膀胱発生機序に関する薬理学的検討" 金沢大学大学院薬学研究科医療薬学専攻、医療薬剤学講座、学位論文, 41 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] KOMATSU,K: "An antisense to NMDA-R1 RELIEVES DECREASE IN BLADDER CAPACITY AFTER FOCAL CEREBRAL INFARCTION IN RATS" NEUROUROLOGY AND URODYNAMICS. VOL16,5. 469 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] YOKOYAMA,O: "EFFECTS OF MK801 ON BLADDER OVERACTIVITY IN RATS WITH CEREBRAL INFARCTION" J.Urology. 159. 571-576 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] YOKOYAMA,O: "CHANGE IN BLADDER CONTRACTILITY ASSOCIATED WITH BLADDER OVERACTIVITY IN RATS WITH CEREBRAL INFARCTION" J.Urology. 159. 577-580 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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