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Molecular Biological Analysis of Invasion and Metastasis in Urothelial Cancer.

Research Project

Project/Area Number 09671630
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionThe University of Tokushima

Principal Investigator

KAGAWA Susumu  University of Tokushima, School of Medicine, Professor, 医学部, 教授 (40035738)

Co-Investigator(Kenkyū-buntansha) YOKOTA Kin-ya  University of Tokushima, University Hospital, Assistant Professor, 医学部附属病院, 講師 (10274218)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsurothelial cancer / invasion / metastasis / matrix metalloproteinase / tissue inhibitor of metalloproteinase / RT-PCR / gelatin zymography / 移転 / MMP-2 / TIMP-2 / MT1-MMP / 膀胱癌 / 遺伝子発現
Research Abstract

Background : Matrix metalloproteinase-2 (MMP-2) which degrades the extracellular matrix or the basement membrane has an essential role in tumor invasion and metastasis. To evaluate the roles of MMP-2, its inhibitor ; tissue inhibitor of metalloproteinases-2 (TIMP-2) and its activator ; membrane-type matrix metalloproteinase-1 (MT1-MMP) on tumor invasion or as a prognostic factor in human bladder cancer, we investigated the expression of MMP-2, TIMP-2, and MT1-MMF in bladder cancers.
Methods : Tissues obtained from 41 patients with bladder cancer were used for analysis. Expression of MMP-2, TIMP-2, MT1-MMP, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Correlations between the levels of MMP-2, TIMP-2, and MT1 -MMP express ion and histologic finding or patient outcome were evaluated.
Results : Expression of MMP-2 and TIMP-2 was significantly higher in muscle invasive pT2 <less than or equal> bladder tumors than in pTa-1 tumors (MMP-2 : p<.0005, TIMP-2 : p<
Moreover, high levels of MMP-2 and TIMP-2, as well as MT1-MMP expression were all strongly associated with decreased survival (MMP-2 : p<.0001, TIMP-2 : p<.0001, MT1-MMP : p<.005). Even within the radically cystectomized muscle invasive pT2 <less than or equal> tumor group, patients with high expression of any of these three genes had a worse prognosis than those with low expression (MMP-2 : p<.05, TIMP-2 : p<.O5, MT1-MMP : p=.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Atsuko Furukawa et al: "Role of the matrix metalloproteinase and tissue inhibitor of metalloproteinase families in noninvasive and invasive tumors transplanted in mice with serere combined immunodeficien" Urology. 51(5). 849-853 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiro-Omi Kanayama et al: "Prognostic values of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in bladder cancer" Cancer. 82(7). 1359-1366 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tsuji et al: "Immunohisto chemical analysis of Ki-67 antigen and Bcl-2 protein expression in prostate cancer:effect of neoadjuvant hormonal therapy" British Journal of Urology. 81. 116-121 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Murakami et al: "MN/C9 gene expression in renal cell carcinona as a potential biomarker evaluated by semi-quantitative RT-PCR" British Journal of Urology. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Atsuko Furukawa, Masahito Tsuji, Masaaki Nishitani, Kazuya Kanda, YoshioInoue, Hiro-omi Kanayama Susumu Kagawa: "Role of the matrix metalloproteinase and tissue inhibitor of metalloproteinase families in noninvasive and invasive tumors transplanted in mice with severe combined immunodeficiency" Urology. 51 (5). 849-853 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiro-omi Kanayama, Kin-ya Yokota, Yasushi Kurokawa, Yoshihide Murakami, Masaaki Nishitani, Susumu Kagawa: "Prognostic values of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in bladder cancer" Cancer. 82 (7). 1359-1366 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tsuji, Murakami, H.Kanayama, T.Sano and S.Kagawa: "Immunohistochemical analysis of Ki-67 antigen and Bcl-2 protein expression in prostate cancer. effect of neoadjuvant hormonal therapy" British Journal of Urology. 81. 116-121 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Murakami, K.Kanda, M.Tsuji, H.Kanayama and S.Kagawa: "MN/C9 gene expression in renal cell carcinoma as a potential biomarker evaluated by semi-quantitative RT-PCR" British Journal of Urology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Atuko Furukawa: "Role of the matrix metallcproteinase and tissue inhibitor of netalloproteinase families in nonivasive and invsive tumoris transplanted in mice with severe combined immunodeficiency" Urology. 51(5). 849-853 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiro-omi Kanayama: "Prognosfic values of matrix metalloprteinase-2 and fisasue inhibitor of metalloproteinase-2 expression in biadder" Cancer. 82(7). 1359-1366 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Tsuji: "Immunohisto caemical analysis of Ki-67 antigen and Bcl-2 protein expression in prostate cancer:effect of neoadjurant hormonal theraph" Briyish Journal ofUrology. 81. 116-121 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 金山博臣: "尿中剥離細胞及び摘出組織を用いた膀胱癌の分子生物学的診断" 臨床泌尿器科. 52(3). 183-192 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.murakami: "MN/S9 gene expression in renal cell carcinoma as a potential biomarker evaluated by semi-quantitatire RT-PCR" British Journal of Urology. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 金山博臣: "Prognostic values of mafrix metalloproteinase2(MMP-2)and tissue inhibitor of metalloproteinase2(TIMP-2)expression in bladder cancer" Cancer. (印刷中).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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