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The role of dopamine D2 receptor in the maintenance of pregnancy

Research Project

Project/Area Number 09671655
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

FURUTA Itsuko  Hokkaido Univ., Med. School., Assist. Pro., 医学部, 助手 (70238682)

Co-Investigator(Kenkyū-buntansha) YAMADA Hideto  Hokkaido Univ., Med. Hospital, Lec., 医学部・附属病院, 講師 (40220397)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsDo amine D2 receptor / Mamtenance for pregnancy / Luteal function / Dopamine agonist / Dopamine
Research Abstract

Luteinization hormone and prolactin secreted by pituitary play an important role in the maintenance of luteal function especially at early pregnancy. It is well known that dopamine agonist (dopamine D2 receptor stimulant) is able to inhibits prolactin release from the pituitary gland. To study the role of dopamine D2 receptor in the maintenance of luteal function and pregnancy, we investigated the effect of cabergoline (CAB), a long-acting dopaminergic agonist , on the steroidogenesis of cultured rat granulosa cells and porcine luteal cells. Also the expression of dopamine D2 receptor mRNA in the mouse ovary was examined by RT-PCR. CAB inhibited the FSH-stimulated production of progesterone by cultured rat granulosa cell at concentrations ranging from 10ィイD1-8ィエD1 to 10ィイD1-4ィエD1M. At high concentrations (10ィイD1-5ィエD1 and 10ィイD1-4ィエD1M), CAB inhibited the production of estradiol by cultured rat granulosa cell. The production of progesterone by cultured luteal cell prepared from porcine corpus luteum was also inhibited by CAB at concentrations ranging from 10ィイD1-8]ィエD1 to 10ィイD1-4ィエD1M in the both condition with or without LH.Ovary from control, under inducing (48 h after injection of PMSG), preovulatory (6h after injection of hCG), ovulatory (12h after injection of hCG), postovulatory(24 and 48 h after injection of hCG) mice was examined for the expression of dopamine D2 receptor mRNA. However, the message of dopamine D2 receptor was not detected in the ovary at any ovulatory cycle These results, that dopamine D2 receptor stimulant directly inhibits the steroidogenesis of granulosa cells and luteal cells in vitro, suggest that dopamine D2 receptor may participate in the maintenance of luteal function and pregnancy.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Noriaki Sakuragi: "Bcl-2 expression and apoptosis in human trophoblast"Trophoblast.Res. 9. 63-74 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideto Yamada: "Low in vitro thyrotropic activity of a human gonadotropin molecule in the first trimester during pregnancy"Gynecol Obstet.Invest.. 46. 75-79 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideto Yamada: "Hematopoietic cytokine levels and in vitro colony formation assay in fetal anemia"Semin Thromb Hemost. 24(5). 485-490 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroaki Negishi: "In vitro production of progesterone by rat granulosa cells regulated by cabergoline and prolactin"Endocrine Res.. 24(2). 195-203 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinichi Tanaka: "Gestational transient hyperthyroxinaemia(GTH) : screening for thyroid function in 23163 pregnant women using dried blood spots"Clin Endocrinol.. 49. 325-329 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsunemaro Koujyo: "Induction of endometriosis and adenomyosis by transvaginal pituitary transplantation in mice with and without natural killer cell activity"Am J Reprod Immunol.. 40(6). 441-446 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideto Yamada: "Fetal treatment of congenital heart block ascribed to anti-SSA antibody : case reports with observation of cardiohemodynamics and review of the literature"Am J Reprod Immunol.. 42. 226-232 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itsuko Furuta: "A Sensitive, Nonradioactive Method for the Detection of a Low Level of Apoptosis in Rat Ovary and Human Placenta. Technical Note"Gynecol Obstet Invest.. 48(4). 259-262 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideto Yamada: "IgA-class anti-beta2-glycoprotein I in women with unexplained recurrent spontaneous abortion"Arthnitis Rheum. 42(12). 2727-2728 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideto Yamada: "Passive immune thrombocytopenia in neonates of mothers with idiopathic thrombocytopenic purpura : incidence and risk factors"Semin Thromb Hemost. 25(5). 491-496 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hideto Yamada: "Factor XII Deficiency in Women with Recurrent Miscarriage"Gynecol Obstet Invest.. 49(2). 80-83 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itsuko Furuta: "Effect of inflammatory cytokines on prostaglandin E2 production from human amnion cells cultured in serum-free condition"Gynecol Obstet Invest.. 49(2). 93-97 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N.Sakuragi: "Bcl-2 e.xpre,ssion and apoptosis in human trophoblast."Trophoblast. Res.. 9. 63-74 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamada: "Low in vitro thyrotropic activity of a human gonadotropin molecule in the. First trimester during pregnancy."Gynecol Obstet Inves. 46. 75-79 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamada: "Hematopoietic cytokine levels and in vitro colony formation assay in fetal anemia."Semin Thrornb Hemost. 24(5). 485-490 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Neghisi: "In vitro production of progesterone by rat granulosa cells regulated by cabergoline and prolactin."Endocrine Res. 24(2). 195-203 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Tanaka: "Gestational lransient hyperthyroxinaemia (GTH) : screening for thyroid ftinction in 23163 pregnant women using dried blood spots."Clin Endocrinol. 49. 325-329 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Koujyo: "Induction of elldometriosis and adenomyosis by transvaginal pituitary transplantation in mice with and without natural killer cell activity."Am J Reprod Immunol. 40(6). 441-446 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamada: "Fetal treatment of congenital heart block ascribed to anti-SSA antibody: case reports with observation of cardiohemodynamics and review of the literature."Am J Reprod Immunol. 42. 226-232 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I. Furuta: "A Sensitive, Nonradioactive Method for the Detection of a Low Leve.1 of Apoptosis in Rat Ovary and Human Placenta. Technical Note."Gynecol Obstet Invest.. 48(4). 259-262 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamada: "IgA-class anti-beta2-g]ycoprotein I in women with unexplained recurrent spontaneous abortion."Arthritis Rheum. 42(12). 2727-2728 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamada: "Passive immune thrombocytopenia in neonates of mothers with idiopathic thrombocytopenic purpura : incidence and risk factors."Semin Thromb Hemost. 25(5). 491-496 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Yamada: "Factor XII Deficiency in Women with Recurrent Miscarriage"Gynecol Obstet Invest. 49(2). 80-83 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I. Furuta: "Effect of inflarnmatory cytokines on prostagiandin E2 production from human amnion cells cultured in serum-free, condition."Gynecol Obstet Invest. 49(2). 93-97 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itsuko Furuta: "A Sensitive, Nonradioactive Method for the Detection of a Low Level of Apoptosis in Rat Ovary and Human Placenta. Technical Note"Gynecol Obstet Invest.. 48(4). 259-262 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideto Yamada: "Fetal treatment of congenital heart block ascribed to anti-SSA antibody : case reports with observation of cardiohemodynamics and review of the literature"Am J Reprod Immunol.. 42(4). 226-232 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Itsuko Furuta: "Effect of inflammatory cytokines on prostaglandin E2 production from human amnion cells cultured in serum-free condition"Gynecol Obstet Invest.. 49(2). 93-97 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideo Yamada: "Factor XII Deficiency in Women with Recurrent Miscarriage"Gynecol Obstet Invest.. 49(2). 80-83 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideto Yamada: "Passive immune thrombocytopenia in neonates of mothers with idiopathic thrombocytopenic purpura : incidence and risk factors"Semin Thromb Hemost. 25(5). 491-496 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideto Yamada: "IgA-class anti-beta2-glycoprotein I in women with unexplained recurrent spontaneous abortion"Arthritis Rheum. 42(12). 2727-2728 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroaki Negishi: "In vitro production of progesterone by rat granulosa cells regulated by cabergoline and prolactin." Endocrine Res.24(2). 195-203 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroaki Negishi: "Intrapenritoncal administration of cytomegalovirus hyperimmunogloblin to the cytomegalovirus-infected fetus." J Perinatol.18(6,Ptl). 466-469 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 古田伊都子: "胎盤におけるアポトーシス" 産婦人科の世界(別冊). 51(1). 37-47 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hideto Yamada: "Low in vitro thyrotropic activity of a human gonadotropin molccule in the first trimester during pregnancy." Gynecol Obstet.Invest.46. 75-79 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shinichi Tanaka: "Gestational transient hyperthyroxinaemia (GTH) : screening for thyroid function in 23163 pregnant women using dried blood spots." Clin Endocrinol.49. 325-329 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsunemaro Koujyo: "Induction of endometriosis and adenomyosis by trransvaginal pituitary transplantation in mice with and without Natural killer cell activity." Am J Reprod Immunol.40(6). 441-446 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroaki Negishi: "Prevention and termination of pregnancy in the rat with cebergoline,a dopamine agonist." J.Reprod.Fertil.109(1). 103-107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroaki Negishi: "Outcome of non-immune hydrops fetalis and a fetus with hydrothorax and/or ascites : With some trials of intrauterine treatment." J Perinat.Med.25(1). 71-77 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐川正: "妊婦の警官粘液中のサイトカイン動態" 産婦人科治療. 75(5). 559-563 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Noriaki Sakuragi: "Bcl-2 expression and apoptosis in human trophoblast." Trophoblast.Res. 9. 63-74 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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