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Molecular cloning of candidate gene for human ovarian carcinogenesis

Research Project

Project/Area Number 09671657
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

ISHIKAWA Mutsuo  Asahikawa Medical College, Department of Obstet. & Gynec., Professor, 医学部, 教授 (20002131)

Co-Investigator(Kenkyū-buntansha) YAGINUMA Yuji  Asahikawa Medical College, Department of Obstet. & Gynec., associate professor, 医学部, 助手 (90250571)
HAYASHI Hiroaki  Asahikawa Medical College, Department of Obstet. & Gynec., associate professor, 医学部, 講師 (20164957)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsuterine / ovary / cancer / p15 / p16 / PTEN / 発癌 / IGF-II / P15 / P16 / Rb
Research Abstract

The mRNA for p14ARF is composed of exons 1b, 2, and 3, whereas the mRNA for p16ィイD2INK4AィエD2 is derived from exons 1a, 2, and 3 . Alternative splicing of exon 1 in p14ィイD2ARFィエD2 results in a different reading frame for exons 2 and 3. Pl4ィイD2ARFィエD2 inhibits the expression of oncogene MDM-2, therefore preventing MDM-2-mediated inactivation of tumor suppressor gene p53. In an effort to analyze molecular mechanisms of human gynecologic carcinogenesis, we studied the structure and expression of the p14ィイD2ARFィエD2 gene. For ovarian cancer cell lines, only small amount of p14ィイD2ARFィエD2 gene expression was observed in PA-1 cell line, and no p14ARF gene was expressed in SK-OV-3 cell line. P14ィイD2ARFィエD2 1b genomic DNA was lost in SK-OV-3 cell line. Loss of p14ィイD2ARFィエD2 gene expression was seen in 10 % (5/46) of ovarian cancer tissues, and p14ィイD2ARFィエD2 1b genomic DNA was lost in 9% (4/46) of ovarian cancer tissues. Low expression was seen in 9% (4/46) of ovarian cancer tissues compared to normal counterparts. On the other hands, for uterine cancers, in all thirteen cell lines and 80 uterine cancer tissues, p14ィイD2ARFィエD2 gene abnormality was not detected using RT-PCR and SSCP analysis. These data suggest that abnormalities of p14ィイD2ARFィエD2 gene may be involved in some human ovarian carcinogenesis, but not in uterine carcinogenesis.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] K.Kawai: "Telomerase Activity and Human Papillomavirus(HPV)Infection in Human Uterine Cervical Cancers and Crvical Cancer"Eur.J.Cancer. 34(13). 2082-2086 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Saitoh: "Analysis of Bcl-2, Bax and Survivin genes in uterine cancer"Inernational Journal of Oncology.. 15. 137-141 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Yamashita: "Codon 72 polymorphism of p53 as a risk factor for patients with human papillomavirus-associated squamous intraepithelial lesions and invasive cancer of the uterine cervix"Carcinogenesis. 20(9). 1733-1736 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yaginuma Y.: "Abnormal structure and expression of PTEN/MMAC1 gene in human uterine cancers"Mol Carcinog.. 27(2). 110-116 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Kawai, Y. Yaginuma, H. Tsuruoka, M. Griffin, H. Hayashi, and M. Ishikawa: "Telomerase Activity and Human Papillomavirus (HPV) Infection in Human Uterine Cervical Cancers and Crvical Cancer."34(13). 2082-2086 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Saitoh, Y. Yaginuma, and M. Ishikawa: "Analysis of Bcl-2, Bax and Survivin genes in uterine cancer."International Journal of Oncology. 15. 137-141 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yamashita, Y. Yaginuma. Y. Saitoh, K. Kawai, T. Kurakane, H. Hayashi, and M. Ishikawa: "Codon 72 polymorphism of p53 as a risk factor for patients with human papillomavirus-associated squamous intraepithelial lesions and invasive cancer of the uterine cervix"Carcinogenesis. 20(9). 1733-1736 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yaginuma Y., Yamashita T., Ishiya T., Morizaki A., Katoh Y., Takahashi T., Hayashi H., and Ishikawa M.: "Abnormal structure and expression of PTEN/MMACI gene in human uterine cancer."Mol Carcinog. 27(2). 110-116 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Kawai: "Telomerase Activity and Human Papillomavirus (HPV) Infection in Human Uterine Cervical Cancers and Crvical Cancer"Eur. J. Cancer.. 34(13). 2082-2086 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Saitoh: "Analysis of Bcl-2, Bax and Survivin genes in uterine cancer"International Journal of Oncology.. 15. 137-141 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Yamashita: "Codon 72 polymorphism of p53 as a risk factor for patients with human papillomavirus-associated squamous intraepithelial lesions and invasive cancer of the uterine cervix"Carcinogenesis. 20(9). 1733-1736 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yaginuma Y.: "Abnormal structure and expression of PTEN/MMAC1 gene in human uterine cancers"Mol Carcinog.. 27(2). 110-116 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuji Yaginuma: "Analysis of the Rb gene and Cyclin-Dependent Kinase 4 Inhibitor Genes(p16^<INK4> and p15^<INK4B>)in Human Ovarian Carcinoma Cell Lines" Experimental Cell Research. 233. 233-239 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yuji Yaginuma: "Relaxation of Insulin-Like Growth Factor-II Gene Imprinting in Human Gynecologic Tumors" Oncology. 54. 502-507 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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