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Study of the cholesterol transportation mechanism in embryo

Research Project

Project/Area Number 09671672
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionShiga University of Medical Science

Principal Investigator

FURUKAWA Hiroyuki  Shiga University of Medical Science, School of Medicine, Assistant Professor, 医学部, 助手 (70293834)

Co-Investigator(Kenkyū-buntansha) NODA Yoichi  Shiga University of Medical Science, School of Medicine, Professor, 医学部, 教授 (50115911)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsmouse embryo / LDL / macrophage / implantation / コレステロール / 胚
Research Abstract

Changes in cell size of preimplantation mouse embryos and the influences of low density lipoprotein on the development of embryos. The total cell volume (TV) in an embryo, calculated by the diameters of cells, decreased of 29.1% but the total cell surface area (TA) did not change during the first cell division. During 2 to 8 cell stage TV was constant but TA increased of 24-26% after each division. Low density lipoprotein (LDL) in the culture media did not improve the blastocyst rates of embryos cultured from 1 cell stage, but significantly increased the area of trophoblast outgrowth cultured after the early blastocyst stage. However the cell surface area incresae during preimplantation embryogenesis, the development improving effect of LDL were observed only after the blastocyst stage.
The concentration and distribution of F4/80-positive macrophages and BM8-positive macro-phages in the uterus of mice during the preimplantation period (between day 1 and 14 of pregnancy) were assessed immunohistochemically. In the endometrium, macrophages were found diffusely on day 0-1 and the number of these cells increased on the lumen side on day 2 and 3.Upon initiation of implantation (day 3-7), significant numbers of macrophages were found on the outside of the decidua formed around the implantation site, but macrophages were not found within the decidua. In the endo-metrium without decidualization, macrophages were found diffusely on day 0-1.Furthermore, this study demonstrates that, during pregnancy, macrophages are not present within the early stage of decidua-lization, but they migrate from a deep layer of the endometrium following the proliferation of the decidua.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] K Wakuda: "Embryo-dependent induction of embryo receptivity of mouse endonetrium" J.Reprod.Fertil. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本 嘉昭: "不妊症患者におけるassisted reproductive technology(ART)適応時期の検討" 産婦人科の進歩. 49(2). 99-105 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 後藤 栄: "In vitro発生停止胚におけるミトコンドリアの挙動・構造とATP量" 産婦人科の進歩. 49(3). 180-192 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 林 嘉彦: "マウスにおける子宮内膜内微少環境修飾による着床率の改善" 産婦人科の進歩. 49(5). 516-527 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 石 紅: "In vitroマウス着床モデルによる脱落膜細胞胚相互作用の解析" 産婦人科の進歩. 50(2). 180-194 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本 嘉昭: "マウス初期胚の細胞体積、表面積の変化と培養液中LDLが胚発生効率に及ぼす影響" 日本受精着床学会雑誌. 15. 183-185 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wakuda K., Takakura K., Nakanishi K., Kita N., Shi H., Hirose M., Noda Y.: "Embryo-dependent induction of embryo receptivity of mouse endometrium." J.Reprod.Fertil.115(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Goto S., Yamade I., et al.: "Morphological studies of mitochondria and ATP level in mouse two-cell block embryos." Adv.Obstet.Gynecol. 49-3. 180-192 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayashi Y., Takakura K., et al.: "Modification of the local milieu surtounding the embryo improves the implantation rate in mice" Adv.Obstet.Gynecol.49-5. 516-527 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirose M., Kita N., et al.: "Embryotrophic factor to mouse preimplantation embryos in culture with somatic cells" Adv.Obstet.Gynecol.49-5. 528-542 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shi H., Takakura K.et al.: "Studies on interaction between decidua and embryo in the mouse deciualization model in vitro" Adv.Obstet.Gynecol.50-2. 180-194 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K Wakada: "Embryo-dependent induction of embryo receptivity of mouse endometrium" J.Repord.Fertil. (In press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 山本 嘉昭: "不妊症患者におけるassisted reproductive technology(ART)適応時期の検討" 産婦人科の進歩. 49(2). 99-105 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 後藤 栄: "In vitro発生停止胚におけるミトコンドリアの挙動・構造とATP量" 産婦人科の進歩. 49(3). 180-192 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 林 嘉彦: "マウスにおける子宮内膜内微少環境修飾による着床率の改善" 産婦人科の進歩. 49(5). 516-527 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 石 紅: "In vitroマウス着床モデルによる脱落膜細胞・胚相互作用の解析" 産婦人科の進歩. 50(2). 180-194 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山本 嘉昭: "マウス初期胚の細胞体積,表面積の変化と培養液中LDLが胚発生効率に及ぼす影響" 日本受精着床学会雑誌. 15. 183-185 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山本嘉明: "マウス初期胚の細胞体積、表面積の変化と培養液中LDLが胚発生効率に及ぼす影響" 第15回日本受精着床学会にて発表.

    • Related Report
      1997 Annual Research Report
  • [Publications] 石川弘伸: "マウス子宮内膜間質細胞を用いたin vitro脱落膜化モデルの作成" 産婦人科の進歩. 49.3. 193-204 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 石 紅: "In vitroマウス着床モデルによる脱落膜細胞・胚相互作用の解析" 産婦人科の進歩. 50.2. 180-194 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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