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Identification of uterine genes induced by embryo during mice implantation.

Research Project

Project/Area Number 09671676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

HIGUCHI Toshihiro  Kyoto University, Gynecology and Obsterics, Assistant, 医学研究科, 助手 (00283614)

Co-Investigator(Kenkyū-buntansha) FUJITA Jun  Kyoto University, Clinical Molecular Biology, Professor, 医学研究科, 教授 (50173430)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsImplanation / Embryo / Endometrium / cDNA subtraction / Infertility
Research Abstract

In this project, we sought to identify the genes involved in the implantation process by subtracting cDNA constructed from the uteri of non-pregnant mice from cDNA from those of pregnancy day 5 mice. One of the identified cDNA encoded the calcium binding protein D-9k (Calbindin). Northern blot analysis showed that the size of the transcript was the same as rat calbindin mRNA, and that it was expressed in the uteri of pregnancy day 5 mice. We found that the expression of calbindin mRNA increased on day 3 of pregnancy when the serum progesterone concentration is high. In fact, progesterone induced a marked increase in the expression of calbindin mRNA.In situ hybridization showed that the uterine expression of calbindin mRNA disappeared in the glandular epithelium on day 5 of pregnancy. In the pseudopregnant mice, the expression of calbindin mRNA was observed in the glandular epithelium on day 5, suggesting that this molecule is regulated not only by the sex steroid hormones, but also by the embryonal signal. To examine this possibility, we performed the embryo transfer experiments. While calbindin mRNA was detected in the glandular epithelium of the control uterine horn, it was not detected in the embryo-transferred horn 36 h after embryo transfer. These results indicated that the expression of calbindin mRNA in the glandular epithelium was suppressed by the presence of blastocysts on day 5 of pregnancy.
In conclusion we could found that embryonic signals modulate the endometrium during implantation. We are now analyzing other cDNA clones cloned from subtracted cDNA library. This approach will contribute towards the clarification of reproductive physiology.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kimihiko,Nakamura: "Integrin α6 is Involved in Follicular Growth in Mice." Biochem Biophys Res Commun. 235. 524-528 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirohiko,Watanabe: "Ovulation defect and its restoration by bone marrow transplantation in osteopetrotic mutant mice of Mitfmi/Mitfmi genotype." Biol Reprod. 57. 1394-1400 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuyuki, Fujita: "Administration of thymocytes derived from non-pregnant mice induces an endometrial receptive stage and leukaemin inhibitory factor expression in the uterus" Hum Reprod. 10. 2888-2894 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Keiji, Tatsumi: "Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation" Mol Hum Reprod. 2. 153-161 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeshi, Hirano: "CD9 is expressd in extravillous trophoblast in association with integrin α3 and integrin α5" Mol Hum Reprod. 2. 162-167 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeshi, Hirano: "CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line. BeWo cells" Mol Hum Reprod. 2. 168-175 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimihiko Nakamura: "Integrin alpha6 is Involved in Follicular Growth in Mice." Biochem Biophys Res Commun.235. 524-528 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirohiko Watanabe: "Ovulation defect and its restoration by bone marrow transplanation in osteopetrotic mutant mice of Mitfmi/Mitfmi genotype." Biol Reprod. 57. 1394-1400 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuyuki Fujita: "Administration of thymocytes derived from non-pregnant mice induces an endometrial receptive stage and leukaemin inhibitory factor expression in the uterus" Hum Reprod. 10. 2888-2894 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Keiji Tatsumi: "Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantaion" Mol Hum Reprod. 2. 153-161 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeshi Hirano: "CD9 is expressed in extravillous trophoblast in association with integrin alpha3 integrin alpha5" Mol Hum Reprod. 2. 162-167 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeshi Hirano: "CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line, BeWo cells" Mol Hum Reprod. 2. 168-175 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuyuki Fujita: "Administration of thymocytes derived from non-pregnant mice induces an endometrial receptive stage and leukaemin inhibitory factor expression in the uterus" Hum Reprod. 10. 2888-2894 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shinya Yoshioka: "Endotheline-Cnoverting Enzyme-1 Is expressed on human ovarian follicles and corpora lutea of menstrual cycle and early pregnancy." J Clin Endocrinol Metab. 83. 3943-3949 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimihiko Nakamura: "Bestatin,an aminopeptidase inhibitor,promotes follicular growth and ovulation suppressed by stress in mice." Endocrine Journal. 45. 547-553 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Keiji Tatsumi: "Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation" Mol Hum Reprod. 2. 153-161 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeshi Hirano: "CD9 is expressd in extravillous trophoblast in association with integrin α3 and integrin α5" Mol Hum Reprod. 2. 162-167 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeshi Hirano: "CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line,BeWo cells" Mol Hum Reprod. 2. 168-175 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimihiko Nakamura: "Integrin α6 is Involved in Follicular Growth in Mice." Biochemical and Biophysical Research Communications. 235. 524-528 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Keiko Takabatake: "Intravenous administration of splenocytes in early pregnancy changes the implantation window in mice." Human Reproduction. 12. 583-585 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirohiko Watanabe: "Ovulation defect and its restoration by bone marrow transplantation in osteopetrotic mutant mice of Mitfmi/Mitfmi genotype." Biology of Reproduction. 57. 1394-1400 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Keiko Takabatake: "Splenocytes in early pregnancy promote embryo implantation by regulating endometrial differentiation in mice." Human Reproduction. 12. 2102-2107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazuyuki Fujita: "Thymocytes promote embryo implantation by regulating endometrial differentiation in mice" Human Reproduction. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shinya Yoshioka: "Membrane-bound carboxypeptidase is expressed on human ovarian follicles and corpora lutea of menstrual cycle and early pregnancy." Human Reproduction. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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