| Project/Area Number |
09671680
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
MORISHIGE Kenichiro Osaka University Medical School, Assistant Professor, 医学部, 助手 (90283788)
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| Co-Investigator(Kenkyū-buntansha) |
山本 敏也 大阪大学, 医学部, 助手 (80283787)
倉智 博久 大阪大学, 医学部, 講師 (40153366)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | potassium channel / pituitary / patch-clamp / TRH / dopamine / TSH / capacitance / RT-PCR / パッチクランプ / TRHドパミン / TSH膜容量 / 電子顕微鏡 |
|---|
| Research Abstract |
G protein-gated inwardly rectifying K+ (K_G) channel, a member of inwardly rectifying K^+ channel, is directly activated by pertussis toxin-sensitive G proteins.In endocrine organs, i.e., the anterior pituitary lobe, it was reported that some neurotransmitters including dopamine and somatostatin hyperpolarize the membrane by activating K_G channels, which results in the inhibition of hormone secretion.We found GIRK1 (Kir3.1), the main subunit of K_G channels, to be localized predominantly on the membranes of intracellular dense core vesicles which contained thyrotropin.Stimulation of these thyrotroph cells with thyrotrpin-releasing-hormone provoked fusion of vesicles to the plasma membrane, increased expression of GIRK1 subunit in the plasma membrane, and markedly enhanced K_G currents stimulated by dopamine and somatostatin.These data indicate a novel mechanism for the rapid insertion of functional ion channels into the plasma membrane, which could form a new type of negative feedback control loop for hormone secretion in the endocrine system.
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