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Involvement of coagulation and fibrinolytic systems and cytokines in placenta with pathogenesis of preeclampsia.

Research Project

Project/Area Number 09671717
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTokyo Women's Medical University

Principal Investigator

NAKABAYASHI Masao  Tokyo Women's Medical University, Medicine, Professor, 医学部, 教授 (70114585)

Co-Investigator(Kenkyū-buntansha) HIRANO Ikuko  Tokyo Women's Medical University, Medicine, MD, 医学部, 助手 (80256601)
SHIOZAKI Mioko  Tokyo Women's Medical University, Medicine, MD, 医学部, 助手 (90226093)
佐倉 まり  東京女子医科大学, 医学部, 助手 (20256613)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordspreeclampsia / placenta / urokinase type plasminogen activator (uPA) / plasminogen activator inhibitor 1 (RAI-1) / thrombomodulin / transforming growth factor-beta (TGF-beta) / fetal fibronectin / uPA / uPAレセプター
Research Abstract

Object : Impaired invasion of the chorionic villi to the basement decidual layer is a possible pathogenesis of preeclampsia. In the process of placentation, the involvement of coagulation and fibrinolytic systems and cytokines in the placenta is suggested. The object of this study is to elucidate the role of coagulation and fibrinolytic systems in the process of placentation in normal and preeclamptic patients.
Methods : We analyzed the fibrinolytic factors - urokinase type plasminogen activator (uPA), uPA receptor (uPAR), plasminogen activator inhibitor-1 (PAI-1), and thrombomodulin (TM) in normal placentae of the 1st, 2nd and 3rd trimester. These results were compared to those of preeclamptic patients.
In addition, transforming growth factor-beta (TGF-beta), which is released by platelet aggregation, was added to cultures of isolated placental cells collected in early pregnancy to examine the effects of TGF-beta on the productions of fetal fibronectin (FFN) and PAI-1.
Results : 1. It wa … More s revealed immunohistochemical by that the localization of uPA and uPAR was recognized in the cytotrophoblastic cells and extravillous trophoblastic cells of the 1st and 2nd trimester placenta.
2. The levels of uPA and uPAR in the 3rd trimester placenta were lower than those in the 1st and 2nd trimester placenta, and there were no differences between those in normal and preeclamptic patients.
3. The levels of PAI-1 in decidual extracts of preeclampsia were significantly higher than those of normal.
4. The levels of TM in chorionic extracts of preeclampsia were significantly lower than those of normal.
5. TGF-beta significantly increased both PAI-1 production in decidual cells and FFN production in chorionic cells in culture in a dose-dependent manner.
Conclusions : 1. These results suggest that expression of uPA and uPAR on trophoblasts may play an important role in the regulation mechanisms of the invasion and implantation of the placenta.
2. It was suggested that the promotion of platelet aggregation and a local increase in TGF-beta in the placenta may contribute to the occurrence of preeclampsia.
3. It was suggested that placental circulation in preeclampsia was in a hypercoagulable and hypofibronolytic state. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Meiyang Gao 他: "The Inbalance of Plasminogen Activators and Inhibitor in Pleeclampsia" J Obstet Gynaecol Res. 22. 9-16 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Meiyang Gao 他: "Preeclampsia Is Associated With Depressed Fibrinolysis in Placenta" J Matern Fetal Invest. 6. 91-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 安藤 一人、他: "妊娠中毒症の発症予防に関する臨床的、基礎的検討 -低用量アスピリン療法を中心として-" 東女医大誌. 67. 1023-1030 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 平野 郁子、他: "胎盤形成過程における線溶系因子の検討" 東女医大誌. 67. 1078-1083 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mari Sakura 他: "Elevated Fetal Fibronectine in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia" J Obstet Gynaecol Res. 24. 73-76 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masao Nakabayashi 他: "Elevated IL-6 in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia" Am J Reprod Immunol. 39. 329-334 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Adachi 他: "Increasingly Safe and Successful Pregnancies" Elsevier Science B.V., 421 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Adachi 他: "Recent Advances on the Pathophysiology of Pregnancy" Simul International, 413 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Meiyang Gao 他: "The Inbalance of Plasminogen Activators and Inhibitor in Pleeclampsia" J Obstet Gynaecol Res. 22. 9-16 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Meiyang Gao 他: "Preeclampsia Is Associated with Depressed Fibrinolysis in Placenta" J Matern Fetal Invest. 6. 91-96 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 安藤 一人、他: "妊娠中毒症の発症予防に関する臨床的、基礎的検討-低用量アスピリン療法を中心として-" 東女医大誌. 67. 1023-1030 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 平野 郁子、他: "胎盤形成過程における線溶系因子の検討" 東女医大誌. 67. 1078-1083 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mari Sakura 他: "Elevated Fetal Fibronectine in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia" J Obstet Gynaecol Res. 24. 73-76 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masao Nakabayashi 他: "Elevated IL-6 in Midtrimester Amniotic Fluid Is Involved with the Onset of Preeclampsia" Am J Reprod Immunol. 39. 329-334 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomoko Adachi 他: "Increasingly Safe and Successful Prognancies" Elsevier Science B.V., 421 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tomoko Adachi 他: "Recent Advances on the Pathophysiology of Pregnancy" Simal International, 413 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakabayashi, M.: "The Imbalance of plsaminogen Activators and Inhibitor in Preeclampsia" J.Obstet.Gynaecol.Res.22(1). 9-16 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Gao, M.: "Preeclampsia Is Associated with Depressed Fibrinolysis in Placenta" J.Matern Fetal Invest. 6. 91-96 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wen-Rong, H.E.: "Geue Expression of endothelin-1 in the Rat Placenta under Chrouic Fetoplacental Hypoxia Induced by Uterine Astery Ligation" J.Tokyo Wom Med.Coll.66(5). 330-337 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakabayashi, M.: "Maternal death and medical complications" Asian Medical Journal. 38(6). 322-327 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyauchi, A.: "Regulation of the plasminogen activator/plasmin system by epidermal growtu factorin cultured numan endometrial cells." Human Reproduction. 10(12). 3284-3288 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakabayashi, M.: "Increasingly Safe and Successful Pregnancies" Elsevier Science B.V., 421 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakabayashi, M.: "Japanese-German Review of Perinatal Medicine" Hokkaido University Press, 322 (1995)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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