| Project/Area Number |
09671739
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
KAMIJO Atsushi Yamanashi Medical University, Department of Otolaryngology, Directuer, 医学部, 助手 (90252022)
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| Co-Investigator(Kenkyū-buntansha) |
TERAKAWA Susumu Hamamatsu Medical University, Professor, 医学部, 教授 (50014246)
MATSUZAKI Zensei Yamanashi Medical University, Department of Otolaryngology, Assistant Professor, 医学部, 講師 (90283217)
OKAMOTO Yoshitaka Yamanashi Medical University, Department of Otolaryngology, Professor, 医学部, 教授 (40169157)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | nitric oxide / airway secretion / mucus / goblet cell / 鼻粘膜上皮杯細胞 |
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| Research Abstract |
NO (nitric oxide) is an important inflammatory mediators that affects many critical cell functions. Recently, high levels of NO production have been reported in the nose or paranasal sinuses. However the role of NO in the nasal airway remains to be clarified. In this study we investigated the effect NO generator (NOC7, NOC5, NOC12) in the mucous secretion from the rat nasal epithelial goblet cells. The amount of mucous secretions were estimated by video enhanced microscopy, this technique enables us to visualize each exocytotic response of the mucous granule from each cell and to count the number of the response. NOC5, NOC7 and NOC 12, the half life is 25 min, 7 min. 100 min respectively (under the condition of 37ﾟC, pH7.4), all increased mucous secretion from the goblet cells. However 6, or 600 muM of NOCS did not increase the exocytotic responses compared with baseline secretion. Eight-bromo-cGMP (3 mM) also increased mucus release. These results suggested that the effect of NO in the secretion from nasal goblet cells limited a narrow dosage, and might activate the guanylate cyclase. SP (substance P) is one of neurotransmitters of sensory C fibers, and known as a potent agonist to the goblet cell secretions. In this study. the effect of SP did not inhibited by L-NAME and L-NNA, indicate that SP and NO might induce the mucous secretion in the different pathway.
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