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Identification of growth factors for neutronal and non-neutronal cells in the rat inner ear

Research Project

Project/Area Number 09671744
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionOsaka University

Principal Investigator

DOI Katsumi  Osaka University Medical School, Assistant Professor, 医学部, 講師 (40243224)

Co-Investigator(Kenkyū-buntansha) TAMURA Manabu  Osaka University Medical School, Assistant Professor, 医学部, 講師 (50273644)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Keywordscochlea / spiral ganglion cell / hair cell / HGF / c-met / RT-PCR / immunohistochemistry / sequence / factor
Research Abstract

RT-PCR and DNA sequencing with rat cochlear cDNAs revealed expressions of mRNAs of hepatocyte growth factor (HGF) and its high-affinity receptor c-met in the rat cochlea. Immunohistochemistry with specific polyclonal antibodies raised against HGF and c-met demonstrated that HGF and c-met proteins were located within the organ of Corti (hair cells and neighboring supporting cells). HGF and c-met proteins were never detected in the Spiral ganglion cells of the normal rats.
Kanamycin-deaf rats were developed by daily kanamycin intra-peritoneal injections after birth. Effects of systemic application of HGF on the survival rates of neuronal and non-neuronal cells were examined. After eight weeks, all hair cells were disappeared in the cochleae of both the HGF-treated and no-HGF deal rats. The number of surviving spiral ganglion cells of the HGF-treated rats was slightly larger than that of the no-HGF deaf rats, although there was no statistical significance between them. In the HGF-treated deaf rats, c-met proteins were detected in the spiral ganglion cells.
These results suggest that HGF and its high affinity receptor c-met are active in the rat cochlea and that they could exert some protective effects on neuronal and non-neuronal cells there.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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