| Project/Area Number |
09671744
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Osaka University |
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Principal Investigator |
DOI Katsumi Osaka University Medical School, Assistant Professor, 医学部, 講師 (40243224)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Manabu Osaka University Medical School, Assistant Professor, 医学部, 講師 (50273644)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | cochlea / spiral ganglion cell / hair cell / HGF / c-met / RT-PCR / immunohistochemistry / sequence / factor |
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| Research Abstract |
RT-PCR and DNA sequencing with rat cochlear cDNAs revealed expressions of mRNAs of hepatocyte growth factor (HGF) and its high-affinity receptor c-met in the rat cochlea. Immunohistochemistry with specific polyclonal antibodies raised against HGF and c-met demonstrated that HGF and c-met proteins were located within the organ of Corti (hair cells and neighboring supporting cells). HGF and c-met proteins were never detected in the Spiral ganglion cells of the normal rats.
Kanamycin-deaf rats were developed by daily kanamycin intra-peritoneal injections after birth. Effects of systemic application of HGF on the survival rates of neuronal and non-neuronal cells were examined. After eight weeks, all hair cells were disappeared in the cochleae of both the HGF-treated and no-HGF deal rats. The number of surviving spiral ganglion cells of the HGF-treated rats was slightly larger than that of the no-HGF deaf rats, although there was no statistical significance between them. In the HGF-treated deaf rats, c-met proteins were detected in the spiral ganglion cells.
These results suggest that HGF and its high affinity receptor c-met are active in the rat cochlea and that they could exert some protective effects on neuronal and non-neuronal cells there.
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