| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
* Nasal gammadelta T cell clones from PAR patients were predominantly CD4-8- and 0D4+and these clones were antigen specific. CD4_ nasal gammadelta T cell clones predominantly expressed the Vgammal/Vdelta-JM gene. CD4+ nasal gammadelta T cell clones expressed increased levels of IL-4, IL-S and IL-13, but negligible IFN-gamma. By contrast, peripheral blood gd T cell clones were 0D8+ and CD4-8-, not antigen specific, Vgamma2fVdelta2 + and expressed increased levels of IFN-gamma.
* NMC from both PAR and CIR patients expressed a variety of cytokines, but significant differences were observed in the proportion of cytokine expressing cells between PAR and CIR in that NMC from PAR patients expressed high levels of IL-4, IL-5, IL-6 and IL-13. Also NMC from PAR patients exhibited increased FcERI expression, cell-bound IgE and IgE-mediated histamine and cytokine release as compared to NMC from CIR patients, even after saturation of the IgE receptors. The density of IgE receptors and IgE molecules in NMC of PAR patients correlated well with the levels of serum igE, and I L-4 upregulated the expression of the FceRl in NMC.Moreover, NMC from PAR patients induced IgE synthesis in B cells. Steroids inhibited the synthesis of IL-4 and 11-13 from nasal mast cells. Taken together, these results and the recently demonstrated IgE-induced upregutation of the FcuRl expression in mast cells suggest critical roles for mast cells in promoting the allergic reaction through an lgE-FcERI-mast cell axis.
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