| Project/Area Number |
09671780
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Hokkaido University |
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Principal Investigator |
KOTAKE Satoshi Medical Hospital., Hokkaido Univ., Lecturer, 医学部・附属病院, 講師 (00186694)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Behcet's disease / Gene cloning / Antigen protein / Streptococci / 連鎮球菌 / 分子相同性 / 免疫反応 |
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| Research Abstract |
We had determined the gene, bes-1, encoding a streptococcal antigen correlated with Behcet's disease. The whole open reading frame of bes-1 consisted of 2550 base pair with a calculated molecular mass of 95 kDa. The aim of this study is to analyze the immunopathologic mechanisms of Behcet's disease. The residues in a portion of the amino acid sequence show 60% correspondence to those of the human intraocular peptide Brn-3b, which is a POU domain expressed in a subset of retinal ganglion cells. For the expression of antigenic protein BES-1, the region spanning the whole sequence of the gene was amplified by PCR technique, and then the PCR product was ligated directly into pGEM-T vector. E.coli transformed with the recombinant plasmid produced a 95 kDa protein that reacted with patient antiserum. We succeeded in the purification of bes-1 using the pET system. Further studies will be needed to evaluate the role of BES-1 in the development of Behcet's disease.
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