| Project/Area Number |
09671802
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | EHIME UNIVERSITY |
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Principal Investigator |
OKAMOTO Shigeki EHIME UNIVERSITY Medical Center, lecturer, 医学部附属病院, 講師 (50274329)
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| Co-Investigator(Kenkyū-buntansha) |
OHASHI Yuichi EHIME UNIVERSITY Shool of Medicine, Chairman, 医学部, 教授 (00116005)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | interleukin 1beta / corneal wound healing / Reumatoid arthritis / MRL / lpr / IL-1 receptor antagonist / 上皮菲薄化 / TNFα / IL-lra(レセプターアンタゴニスト) / MRLlpr |
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| Research Abstract |
1) Excessive expression of IL-113 mRNA in MRL/Ipr strain of mouse, that is a model mouse of Rheumatoid arthritis. IL-1BETA expression in the cornea of RA model mice were investigated with RT-PCR and immunohistochemistry technique. Interleukin lBETA was excessively expressed only in the cornea of RA model mouse.
2) The wound healing of the cornea was delayed in RA model mouse
The cornea wound was made by photoabrasion with EXCIMER laser. The wound healin of the cornea in RA model mouse was significantly delayed compared to that in BALB/c mice.
3) The IL-1BETA level in the tear of RA patients was significantly higher than that in the teat of healthy volunteers.
Interleuikin-lBETA level of tear was measured by ELISA.The IL-lBETA level in tears of RA patients was 2370pg/ml, but that in tears of healthy volunteers was 82.7pg/mL
4) Epithelial thinning in RA model mouse
The thickness of RA model mouse is significantly thinner than that of non-RA mouse.
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