• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Ocular Surface reconstruction for cicatricial disorders using amniotic membrane.

Research Project

Project/Area Number 09671819
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

SHIMAZAKI Jun  Tokyo Dental College, Department of Dentistry, Lecturer, 歯学部, 講師 (40170930)

Co-Investigator(Kenkyū-buntansha) SHINOZAKI Naoshi  Tokyo Dental College, Department of Dentistry, Lecturer, 歯学部, 講師
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsfibroblasts / conjunctival epithelium / amniotic membrane / growth factors / cell proliferation / Ocular Surface / cicatricial keratoconjunctivitis / dry eye
Research Abstract

1. Histology and preservation : Fresh human amniotic membrane (AM) had uniform thickness of collagen layer and overlying epithelial cells. The epithelial cells lost their viability up to 80% after 70 days' cryopreservation with DMSO.
2. Animal modesl : Human preserved AMs were secured on bare sciera of albino rabbits and histological changes were studied. The amniotic epithelium remained for about one week postoperatively, then gradually replaced by host conjunctiva. Inflammatory cells were observed beneath the membrane in some cases.
3. Clinical samples : AMs were recognized in 3 out of 14 human samples which had reoperation following AM transplantation, suggesting AMs were not degraded in some cases after transplantation.
4. Growth factors : Roles of growth factors on proliferation/differentiation of ocular surface epithelia were studied using immunohitochemistry and ELISA.bFGF, HGF, and TGF-beta3 were found primarily in the cytoplasm of AM epithelium, whereas TGFbeta 1 and TGF-beta3 were distributed more diffusely. Concentrations of bFGF, HGF, TGF-betal, TGF-beta3 in AM were 5.31*3.04, 14.2*6.0, 2.45*1 .45.0.56*0.45 ng/g, respectively. The concentrations decreased to 1 7.7-23.5% after 70 days' cryopreservation.
5. Effects on ocular surface cells : Addition of homogenized AMs to either cultured conjunctival epithelium or subccnjunctival fibroblasts resulted in significant increases in proliferation of these cells.
6. Cultivation of conjunctival epithelium on AM : We cultivated human conjunctival epithelium on AM using SHEM and RCGM media. These cells became multilayered with intercellular complex formation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Shimazaki J: "Transplantation of amniotic membrane and limbal autograft for patients with recurrent pterygium associated with symblepharon" Br J Ophthalmol. 82. 235-240 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 久保寺秀徳: "羊膜移植後の組織学的検討" 日眼会誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J: "Role of Basic FGF, HGF, and TGF-b in Transplantation of Human Amniotic Membrane." Br J Ophthalmol. (投稿予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 島崎潤: "羊膜移植の考え方" 眼科手術. 11. 429-434 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] "眼科診療Q&A" 六法出版社, (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J,Shinozaki N,Tsubota K.: "Transplantation of amniotic membrane and limbal autograft for patients with recurrent pterygium associated with symblepharon." Br J Ophthalmol. 82. 235-240 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J.: "Concent of amniotic membrane transplantation." Jpn J Ophthalmic Surg. 11. 429-434 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kubodera H,Sato H,Shimazaki J,Tsubota K.: "Pathological changes after amniotic membrane transplantation." Jpn J Ophthal Soc. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J,Shinozaki N,Sato H,Shimmura S,Tsubota K.: "Role of Basic FGF, HGF, and TGF-b in Transplantation of Human Amniotic Membrane." Br J Ophthalmol. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato H,Shimazaki J,Shinozaki N,Tsubota K.: "Role of growth factors for ocular surface reconstruction after amniotic membrane transplantation." Invest Ophthalmol Vis Sci. (suppl) 39 : # 1989. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kubodera H,Sato H,Shimazaki J,Tsubota K.: "Histological changes after preserved human amniotic membrane in rabbitis." Invest Ophthalmol Vis Sci. (suppl) 39 : #1994. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J,Sato H,Shinozaki N,Tsubota K.: "Ocular surface reconstruction for cicatricial keratoconjunctivitis using amniotic membrane." 2nd Meeting of Dermotological Research. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishioka M,Yagi Y,Fujita H,Shimazaki J,Tsubota K.: "Impression cytology of conjunctiva after amniotic membrane transplantation." The 22th Japan Cornea Conference.(1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J.: "Ocular surface reconstruction for cicatrical disorders using amniotic membrane." 264th Biannual Meeting of the Tokyo Dental College. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki, J.: "Amniotic membrane transplantation for cicatricial keratoconjunctivitis." Placenta Association.(1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki, J.: Indication and surgical methods of amniotic membrane transplantation.Ophthalmology Clinic Q&A.Roppo Publisher, 583, 516-517 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J.: Changes in conjunctiva caused by chemical burn.Ophthalmology Clinic Q&A.Roppo Publisher, 521, 522-523 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimazaki J: "Amniotic membrane transplantation for ocular surface reconstruction in patients with chemical and thermal burus." Ophthalmology. 104. 2068-2076 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shimazaki J: "Transplantation of amniotic membrane and limbal autograft for patients with tecurrent pterygium associated with symbrepharon." Br J Ophthalmol. 82. 235-240 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujishima H: "Trabeculectomy with the use of amniotic membrane for uncoutrollable glaucoma." Ophthalmic Surg and Lasers. 29. 428-431 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 島崎 潤: "羊膜移植の考え方" 眼科手術. 11. 429-434 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 島崎 潤: "眼科診療Q&A" 六法出版社, 2 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shimazaki J.: "Amniotic membrane transplantation for ocular surface reconstruction in patients with chemical and thermal burns." Opthalmology. 104,12. 2068-2076 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi