| Project/Area Number |
09671824
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kinki University School of Medicine |
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Principal Investigator |
MISHIMA Hiroshi Kinki University, School of Medicine, Department of Ophthalmology, Associate Professor, 医学部・附属病院, 助教授 (80199984)
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| Co-Investigator(Kenkyū-buntansha) |
HIBINO Tsuyoshi Kinki University, School of Medicine, Department of Ophthalmology, Fellow, 医学部, 講師 (60288917)
阿部 孝助 近畿大学, 医学部, 助手 (00222646)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | SPARC / Cornea / Collagen gel / wound healing / α smooth muscle action / TGF-β / コラーゲンゲル収縮 / αSMA |
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| Research Abstract |
When corneal keratocytes were embedded in a collagen gel and cultured with SPARC (secreted protein which is acidic and rich in cysteine), the collagen gel contraction by keratocytes was stimulated by SPARC in a dose dependent manner. Keratocytes on a plastic plate showed cell contraction when SPARC was added to the medium. The number of the cells expressing α-smooth muscle action (αSMA) significantly increased by the addition of SPARC. The amount of αSMA in 100,000 cells also increased. In immunohistrorical study, SPARC was found in the endothelium of the normal cornea. On the other hand, SPARC was found in the epithelium and the stroma of the corneal wound. The stimulatory effect of SPARC on the collagen contraction by keratocytes was inhibited by the anti-TGFβ antibody. TGFβ (transforming growth factor β) stimulated the collagen contraction by keratocytes and this effect was inhibited by LAP (latency associated peptide). SPARC antagonized the inhibitory effect of LAP on TGFβ. SPARC was found to bind to LAP in the culture medium. These findings indicated that SPARC played an important role on the corneal wound healing.
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