| Project/Area Number |
09671838
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | Keio University |
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Principal Investigator |
MORIKAWA Yasuhide KEIO UNIVERSITY, SCHOOL OF MEDICINE, LECTURER, 医学部, 講師 (90124958)
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| Co-Investigator(Kenkyū-buntansha) |
吉岡 成浩 慶應義塾大学, 医学部, 助手 (60276285)
星野 健 慶応義塾大学, 医学部, 助手 (70190197)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | ENDOSCOPIC SURGERY / FETOSCOPY / TRACHEA / FETAL SURGERY / DIAPHRAGMATIC HERNIA / KERATINOCYTE GROWTH FACTOR / SURFACTANT PROTEIN / HYPOPLASTIC LUNG / 内視鏡下手術 / 先天性横隔膜ヘルニア / KGF-r / nitrofen |
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| Research Abstract |
Endoscopic tracheal ligation in utero was attempted in 6 fetal goat at various gestational ages from 90 to 145 days (term-145days). Using CO2 insufflation and gentle holding of the fetal head made fetoscopic tracheal ligation possible, however, fetuses resulted in still birth after 48-72 hours. The fetal lung was expanded almost twice in weight by tracheal ligation. Eventual tracheal laceration caused prematurity of the fetal lung. USG guided tracheal puncture to obstruct trachea seems to be less invasive because the longest survival up to 144 hours was obtained.
Nitrofen induced CDH (congenital diaphragmatic hernia) revealed impaired expression of mRNA for Keratinocyte Growth Factor at day 20 embryo of SD rats while SP-C and KGFR were the same level. The data was obtained by RT-PCR, however, the result was not reproducible. Further investigation by the northern blot analysis is now on the way.
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