| Project/Area Number |
09671842
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAMAMOTO Tsuneyuki School of Dent., Hokkaido Univ., Asso.Pro, 歯学部, 助教授 (80200822)
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| Co-Investigator(Kenkyū-buntansha) |
DOMON Takanori School of Dent., Hokkaido Univ., Inst., 歯学部, 助手 (50217618)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Cellular cementum / Cementoblast / Sharpey's fiber / Principal fiber / Intrinsic fiber / シャーピ-線維 |
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| Research Abstract |
This study observed cellular cementum in human premolars by light and electron microscopy to elucidate how Sharpey's fibers are embedded by the intrinsic fibers. For scanning electron microscopy, KOH-collagenase method was applied to remove elements other than cells.
Where Sharpey's fibers were dense, cementoblasts had wing-like processes encircling the principal fibers. On the cementum-facing side, wing-like processes were segmented into finger-like processes in parallel with the cementum surface. Where Sharpey's fibers few or absent, cementoblasts had only a few or no wing-like processes, but had finger-like processes on the cementum-facing side. The main results suggest that : there are two kinds cementoblasts. One has finger-like processes associated with wing-like processes and secrete intrinsic fibers from the finger-like processes to incorporate the principal fibers. The second type has finger-like processes not associated with wing-like processes, this type secretes intrinsic fibers from the finger-like processes to adjust the cementum thickness.
In rat acellular cementum, cementum-forming cells had wing-like processes encircling the principal fibers. Finger-like processes extended toward the cementum. The arrangement of finger-like processes may result in the formation of acellular cementum containing no intrinsic fibers.
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