| Project/Area Number |
09671843
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SUGAWARA Shunji School of Dentistry, Tohoku University, Assistant Professor, 歯学部, 助手 (10241639)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Akiko School of Dentistry, Tohoku University, JSPS Fellow, 歯学部, 日本学術振興会特別研
RIKIISHI Hidemi School of Dentistry, Tohoku University, Lecturer, 歯学部, 講師 (70091767)
TAKADA Haruhiko School of Dentistry, Tohoku University, Professor, 歯学部, 教授 (30135743)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | black-pigmented bacteria / human gingival epithelial cells / cytokines / adhesion molecules / activation mechanism |
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| Research Abstract |
1. Human gingival epithelial cells produce interleukin (IL)-8, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in response to Prevotella intermedia glycoprotein (PGP), and fimbriae andl ipopolysaccharide (LPS) fraction of Porphyromonas gingivalis. In contrast, the cells produce macrophage-colony stimulating factor stimulated with interferon (IFN)-gamma.
2. P.intermedia PGP, fimbriae and LPS fraction of P.gingivalis augmented the expression of intercellular adhesionmolecule-1 (ICAM-1) on the surface of human gingival epithelial cells, flow cytometically.
3. mRNA induction of the above cytokines and ICAM-1 in the cells by the components of black-pigmented bacteria (BPB)was confirmed by reverse-transcriptase PCR.
4. Human gingival epithelial cells do not express CD14, a major LPS receptor, which indicates that the cells are activated by the components of BPB with a distinct pathway from LPS of Enterobacteriaceae. In 1998, Toll-like receptors (TLRs)were reported to be signaling molecules of UPS, and also TLRs mediated LPS signal in a CD14-independent manner at a high concentration of LPS.The experiment that TLRs participate in the activation of human gingival epithelial cells by components of BPB is under way.
5. Human gingivalepithelial cells possess IL-18, a IFN-gamma inducing factor, in the cells as a possibly precursor form. Thes timulants used in this study could not induce the secretion of IL-18 by the cells. However, it is possible that IL-18produced by the cells with some stimulation, such as apoptotic pathway may induce IFN-gamma by immune cells, and that in turn, IFN-gamma produced may participate in the onset and cure of inflammation in periodontium.
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