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Morpho-functional study of nitric oxide synthase-positive neurons in the brain stem that are involved in oral sensori-motor function.

Research Project

Project/Area Number 09671852
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOsaka University

Principal Investigator

TAKEMURA Motohide  Osaka University Faculty of Dentistry, Osaka University, Assistant Professor, 歯学部, 講師 (70192169)

Co-Investigator(Kenkyū-buntansha) NAGASE Yoshitaka  Osaka University Faculty of Dentistry, Osaka University, Research Associate, 歯学部, 助手 (50252698)
YONEHARA Norifumi  Osaka University Faculty of Dentistry, Osaka University, Assistant Professor, 歯学部, 講師 (70124534)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsnitric oxide / NADPH-diaphorase / trigeminal nucleus / sensory / motor / regeneration / neurotoxicity / pain / 三叉神経核 / 感覚 / 運動 / 孤束核 / 疼痛
Research Abstract

Neurons that express neuronal nitric oxide synthase (nNOS) or Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) are considered to produce nitric oxide (NO) on demand. NADPH-d activity and the spatial distribution were characterized histochemically with nerve insults and the WGA-HRP-labeled central terminal fields from the intra- and peri-oral afferents. In the subnucleus caudalis (SpVc), most labeled neurons were in the superficial zone, and smaller numbers were in the magnocellular zone. The NADPH-d-positive neurons in the SpVo/Sn and the dorsomedial SpVc overlapped mostly with the transganglionically labeled terminal field from the lingual nerve, partly with the terminal field from the inferior alveolar, mental and chorda tympani nerves. A statistically significant reduction in the number of NADPH-d-positive neurons was seen bilaterally in SpVo/Sn when the lingual nerve was transected. Inflammatory insults to the lingual nerve and/or tooth pulps significantly increased … More the number of NADPH-d-positive neurons in SpVo/Sn, and SpVc. Thus NADPH-d-positive neurons in the SpVo/Sn and SpVc could receive and be regulated by primary afferent inputs from intra-oral structures especially from the tongue. Activity of nNOS that expressed in primary neurons after nerve transection is involved in nerve regeneration. Because systemic administration of L-nitro arginine methyl ester (L-NAME; NOS blocker) decreased the number of regenerated inferior alveolar nerve into the tooth pulp. Injection of complete Freund's adjuvant (CFA) into hind foot pad induces prolonged hyperalgesia for more than one week. Three days after injection of CFA the number of NADPH-d or NOS-positive neurons increased in the L4-5 dorsal horn. Administration of L-NAME reduced adjuvant induced prolonged thermal hyperalgesia but not normal nociception and acute hyperalgesia. Thus increased NO in the spinal dorsal horn could be involved in maintenance of hyperalgesia and/or prolonged pathological nociception. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] 竹村元秀: "三叉神経核と一酸化窒素"ブレインサイエンス. 8. 895-901 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishikawa N.: "Endothlins promote the activation of astrocytes in rat neostriatum through ET_B receptors"European Journal of Neuroscience. 8. 895-951 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonehara N.: "Involvement of nitric oxide in re-innervation of rat molar pulp following transection of the inferior alveolar nerve"Brain Research. 757. 31-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida A.: "Organization of the descending projection from the parabrachial nucleus to the trigeminal sensory nuclear complex and spinal dorsal horn in the rat"Journal of Comparative Neurology. 383. 94-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagase Y.: "Serotonergic axonal contacts on identified cat trigeminal motoneurons and their correlation with medullary raphe nucleus stimulation"Journal of Comparative Neurology. 384. 443-455 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bae Y. C.: "Identification of-aminobutyric acid-immunoreactive axon endings associated with mesencephalic periodontal afferent terminals and morphometry of the two types of terminals in the cat supratrigeminal nucleus"Journal of Comparative Neurology. 389. 127-138 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakagawa S.: "Ultrastructural observations of synaptic connections of vibrissa afferent terminals in cat principal sensory nucleus and morphometry of related synaptic elements"Journal of Comparative Neurology. 390. 12-33 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonehara N.: "Nitric Oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia"Japan Journal of Pharmacology. 75. 327-335 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishimoto H.: "Central distribution of synaptic contacts of primary and secondary jaw muscle apindle afferents in the trigeminal motor nucleus of the cat"Journal of Comparative Neurology. 391. 50-63 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwase K.: "Ontogeny of NADAH-diaphorase in rat forebrain and midbrain"Anatomy and Embryology. 197. 229-247 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats"Brain Research. 781. 78-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Moritani M.: "Morphological differencies between fast and slowly adapting lingual afferent terminations in the principal and oral nuclei in the cat"Journal of Comparative Neurology. 396. 64-83 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koyama Y.: "BQ788, an endothelin ET_B receptor antagonist, attenuates stab wound injury-induced reactive astrocytes in rat brain"Glia. 26. 268-271 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimo T.: "Connective tissue growth factor induces the proliferation, migration and tube formation of vascular endothelial cells in vivo and angiogenesis in vivo"Journal of Biochemistry. 126. 137-145 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takemura M.: "Mapping of c-Fos in the trigeminal sensory nucleus following high- and low-intensity afferent stimulation in the rat"Experimental Brain research. 130. 113-123 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Kuroda: "Somatosensory cortex stimulation-evoked analgesia in rats : potentiation by NOS inhibitor"Life Science. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takemura M.: "Neurobiology of Mastication from Molecular to Systems Approach"Elsevier. 76-78 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishikawa N.: "Endothelins promote the activation of astrocytes in rat neostriatum through ETィイD2BィエD2 receptors"Eur. J. Neurosci.. 9. 895-901 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonehara N.: "Involvement of nitric oxide in re-innervation of rat molar pulp following transection of the inferior alveolar nerve."Brain Res.. 757. 31-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida A.: "Organization of the descending projection from the parabrachial nucleus to the trigeminal sensory nuclear complex and spinal dorsal horn in the rat."J. Comp. Neurol.. 383. 94-111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagase Y.: "Serotonergic axonal contacts on identified cat trigeminal motoneurons and their correlation with medullary raphe nucleus stimulation."J. Comp. Neurol.. 384. 443-455 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bae Y. C.: "Identification of_-aminobutyric acid-immunoreactive axon endings associated with mesencephalic periodontal afferent terminals and morphometry of the two types of terminals in the cat supratrigeminal nucleus."J. Comp. Neurol.. 389. 127-138 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakagawa S.: "Ultrastructural observations of synaptic connections of vibrissa afferent terminals in cat principal sensory nucleus and morphometry of related synaptic elements."J. Comp. Neurol.. 390. 12-33 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia."Jap. J. Pharmacol.. 75. 327-335 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishimoto H.: "Central distribution of synaptic contacts of primary and secondary jaw muscle spindle afferents in the trigeminal motor nucleus of the cat."J. Comp. Neurol.. 391. 50-63 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwase K.: "Ontogeny of NADAH-diaphorase in rat forebrain and midbrain."Anat. Embryol.. 197. 229-247 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats."Brain Res.. 781. 78-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Moritani M.: "Morphological differences between fast and slowly adapting lingual afferent terminations in the principal and oral nuclei in the cat."J. Comp. Neurol.. 396. 64-83 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koyama Y.: "BQ788, an endothelin ETィイD2BィエD2 receptor antagonist, attenuates stab wound injury-induced reactive astrocytes in rat brain."Glia. 26. 268-271 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimo T.: "Connective tissue growth factor induces the proliferation, migration and tube formation of vascular endothelial cells in vitro and angiogenesis in vivo."J. Biochem.. 126. 137-145 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takemura M.: "NADPH-diaphorase in the brainstem trigeminal sensory nuclear complex. Neurobiology of Masticatioication from Molecular to Systems Approach."Elsevier. 76-78 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takemura M.: "Mapping of c-Fos in the trigeminal sensory nucleus following high-and low-intensity afferent stimulation in the rat."Exp. Brain Res.. 130. 113-123 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Kuroda: "Somatosensory cortex stimulation-evoked analgesia in rats : potentiation by NOS inhibitor."Life Sci.. (In press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Koyama Y.: "BQ788, an endothelin ET_B receptor antagonist, attenuates stab wound injury-induced reactive astrocytes in rat brain"GLIA. 26. 268-271 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimo T.: "Connective tissue growth factor induces the proliferation, migration and formation of vascular endothelial cells and angiogenesis"Journal of Biochemistry. 126. 137-145 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takemura M.: "Mapping of c-Fos in the trigeminal sensory nucleus followng high-and low-intensity afferent stimulation in the rat"Experimental Brain Research. 130. 113-123 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] R.Kuroda: "Somatosensory cortex stimulation-evoked analgesia in rats: potentiation by NOS inhibitor"Life Science. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takemura,M.: "Neurobiology of Mastication from Molecular to Systems Approach"Elsevier. 227-240 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iwase K.: "Ontogeny of NADAH-diaphorase in rat forebrain and midbrain." Anatomy and Embryol.ogy. 197. 229-247 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity inthe trigemino-solitary complex of rats." Brain Research. 781. 78-90 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Moritani M.: "Morphological differences between fast and slowly adapting lingual afferent terminations in the principal and oral nuclei in the cat." Journal of Comparative Neurology. 396. 64-83 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takemura M.: "NADPH-daphorase in the developing rat : Lower brainstem and cervical spinal cord with special reference to the trigemino-solitary complex." Journal of Comparative Neurology. 365. 511-525 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wakisaka S.: "Neuropeptide Y-immunoreactive primary afferents in the dental pulp and periodontal ligament following nerve injury to the inferior alveolar nerve in the rat." Brain Research. 712. 11-18 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Donovan D.M.: "Transgenic mice : Preproenkephalin and the mu opiate receptor." Annals New York Academy of Science. 780. 19-28 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Bae Y.C.: "Electron microscopic observations of synaptic connections of jaw-muscle spindle and periodontal afferent terminals in the trigeminal motor and supratrigeminal muclei in the cat." Journal of Comparative Neurology. 374. 421-435 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 竹村元秀: "三叉神経核と一酸化窒素:2.特集・神経系の一酸化窒素(監修:俣野)" ブレインサイエンス. 8・2. 39-47 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishikawa N.: "Endothelins promote the activation of astrocytes in rat neostriatum through ET_B receptors." European Journal of Neuroscience. 9. 895-901 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonehara N.: "Involevement of nitric oxide in re-innervation of rat molar pulp following transection of the inferior alveolar nerve." Brain Research. 757. 31-36 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida A.: "Organization of the descending projection from the parabrachial nucleus to the trigeminal sensory nuclear complex and spinal dorsal horn in the rat." Journal of Comparative Neurology. 383. 94-111 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagase Y.: "Serotonergic axonal contacts on identified cat trigeminal motoneurons and their correlation with medullary rephe nucleus stimulation." Journal of Comparative Neurology. 384. 443-455 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Bae Y.C.: "Identification of gamma aminobutyric acid immunoreactive axon endings associated with mesencephalic periodontal afferent terminals and morphometry of the two types of terminals in the cat supratrigeminal nucleus." Journal of Comparative Neurology. 388. 421-435 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakagawa S.: "Ultrastructural observations of synaptic connections of vibrissa afferent terminals in cat principal sensory nucleus and morphometry of related syaptic elements." Journal of Comparative Neurology. 389. 12-33 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kishimoto H.: "The differences in central projection and spatial distribution of synaptic contacts on motoneurons between two physiologically defined groups of cat jaw-muscle spindle afferents." Journal of Comparative Neurology. 391. 50-63 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia." Japanese Journal of Pharmacology. 75. 327-335 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iwase K.: "Ontogeny of NADPH-diaphorase in rat forebrain and midbrain." Anatomy and Embryology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats." Brain Research. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia." Japanese Journal of Pharmacology. 75. 327-335 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iwase K.: "Ontogeny of NADPH-diaphorase in rat forebrain and midbrain." Anatomy and Embryology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats." Brain Research. (in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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