| Project/Area Number |
09671871
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
ISHIHARA Kazuyuki Tokyo Dental College, Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (00212910)
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| Co-Investigator(Kenkyū-buntansha) |
KIMIZUKA Ryuta Tokyo Dental College, Faculty of Dentistry, Assistant, 歯学部, 助手 (90287178)
YAMANAKA Ayumi Tokyo Dental College, Faculty of Dentistry, Assistant, 歯学部, 助手 (40231667)
MIURA Tadashi Tokyo Dental College, Faculty of Dentistry, Assistant, 歯学部, 助手 (10266570)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Protease / Hemolytic activity / Treponema denticola / Fimbriae / Expression regulation / Actinobacillus actinomycetemcomitans / Pathogenicity / Bacteroides forsythus / fimbriae / hemolytic activity / expression regulation / Cloning / Fimhrial / Sequence / Treponema denticola / Actinbacillus actinomycetecom / Bacteroides forsythus |
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| Research Abstract |
Adult periodontitis was Infectious disease and it induce tooth loss. It cause a mastication problem and finally induce poor quality of life in patients with periodontal disease. As many attempts to clarify pathogen of the disease have been performed, the molecular mechanisms by which these pathogens exert their effects are poorly understood. To clarify the pathogenic mechanism of potential periodontopathic bacteria, we tried to characterize genes encoding pathogens from Actionbacillus actinomycetemcomitans, Bacteriodesforsydius and Treponema denticola.
We determined the fimbriae gene (fap) of A.actinomycetemcomitans to characterize its role in colonization. The fimbriated strain of this microorganism lost fimbriae upon passaging onto solid medium. W found that expression of the fap gene was decreased in non-fimbriated strain (Microbiol. Pathog. 1997, 23 : 63-69).
Proteases were known to exert a cytotoxic effects to host tissue. We detennined the sequence of a protease gene (prH) of B.for
… More
sythus which is difficult to get enough growth in synthetic medium for a characterization. A molecular mass of the protease is 47,873 and the PrtH possessed hemolytic activity (Imfect. Immun. 1997, 65 : 4888-4891).
Several reports indicated the systemic chemotherapy failure was caused by colonization of Helicobcterpylori in oral cavity. We evaluated the antagonistic effects between oral microorganism and H.pylon. H.pylon was coaggregated with P.gingivalis and F.nucreatum. On the other hand, several oral bacteria inhibits growth of H.pylon (FEMS Microbial. Lett. 1997, 152 : 355-361).
Evaluation of a pathogenicity using wild type strain and single gene deficient mutant is the most effective way to evaluate its pathogenicity. We constructed a knockout mutant of the prolyl-phenylalanine specific protease (dentilisin) in T denticola and characterized the mutant. Surface hydrophobicity of this microorganism decreased dramatically and ability of abscess formation decreased in the mutant. In addition, we found that dentilisin is associated with organization of outer sheath protein. These results indicated that dentilisin exert a cytotoxic effects to host tissue directly (J.Bacteriol. 1998, 180 : 3837-38449). Less
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