Analysis of T cell apoptosis induced by volatile fatty acids
| Project/Area Number |
09671872
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
OCHIAI Tomoko NIHON UNIVERSITY,SCHOOL OF DENTISTRY AT MATSUDO,INSTRUCTER, 松戸歯学部, 助手 (20130594)
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| Co-Investigator(Kenkyū-buntansha) |
OCHIAI Kuniyasu NIHON UNIVERSITY,SCHOOL OF DENTISTRY AT MATSUDO'ASSISTANT PROFESSOR, 松戸歯学部, 講師 (50095444)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | APOPTOSIS / T CELLS / VOLATILE FATTY ACID / BCL-2 / BAX / FAS / P53 / CASPASE / bci-2 / P53 / Caspase |
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| Research Abstract |
(1) Volatile fatty acids, especially butyric acid, metabolic by-products of periodontopathic bacteria, induced apoptosis in murine thymocytes, splenic T cells, human peripheral blood mononuclear cells (PBMC), and Jurkat cells. The apoptosis sensitivity of these cells to butyric acid was thymocytes > Jurkat cells> splenic T cells> PBMC.
(2) Western blotting analysis which was used to study the expression of bcl-2, Bax, Fas and FasL, involved in the response to DNA damage and the induction of apoptosis, indicated that expression of bcl-2 was reduced and phosphorylated bcl-2 was also disappeared in butyric acid-treated cells. No changes in Bax, Fas and Fas-L was demonstrated. FACS analysis revealed that butyric acid suppressed the ratio of bcl-2^+ cells but not of Fas^+ cells.
(3) RT-PCR and Western blotting analysis indicated that there was no increase in p53 expression in butyric acid-induced T cell apoptosis. The DNA fragmentation assay indicated that T cell blasts derived from mice containing a germ-line deficiency of the p53 tumor suppressor gene were susceptible to butyric acid-induced apoptosis to the same degree as wild-type T cells.
(4) Annexin V binding experiments in flow cytometly revealed that butyric acid increased the number of early- and late-stage apoptotic cells.
(5) Butyric acid-induced T cell apoptosis was accompanied by Caspase 3 and Caspase 6 protease activities but not by Caspase 1 and Caspase 8 protease activities.
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Report
(3 results)
Research Products
(6 results)
