| Project/Area Number |
09671876
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
YOSHIMOTO Hisashi Kanagawa Dental College, associate professor, 歯学部, 助教授 (60084787)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Yusuke Kanagawa Dental College, assistant, 歯学部, 助手 (20267511)
HAMADA Nobushiro Kanagawa Dental College, assistant, 歯学部, 助手 (20247315)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Porphyromonas / gingivalis / fimbriae / fimbrillin / transformation / fimA / fimbriae / transformation / フィンブリリン / fimA / 遺伝子 |
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| Research Abstract |
In this study, we demonstrated the successful transformation of Porphyromonas gingivalis with fimA, the gene for the major subunit protein of the fimbriae from strain 381using a newly developed host-vector system for this species including a plasmid vector pYH420 and a restriction-deficient host strain YH522. With this strain, expression of the introduced fimA gene resulted in production of fibrous structures which were serologically distinguishable from the host's endogenous fimbriae by immunogold electron microscopy.
The plasmid DNA carrying the cloned fimA gene was prepared from YH522 transformants and used for transformation of other P. gingivalis strains. Transformants were obtained from six different hosts and compared for their fimbrial expression as well as the capacity for adhesion relative to their respective parental strains. Although a considerable diversity in the expression level of the recombinant fimbriae was observed among those transformants, it was evident that the strains expressing higher levels of the recombinant fimbriae exhibited greater decrease in adhesion activity. This appeared to be related to the evidence that the expression of the host's endogenous fimbrillin was somehow suppressed by recombinant fimA-gene expression in the transformants.
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