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Antibiotic resistance of an endodotitis-associated bacterium, Porphyromonas endodontalis.

Research Project

Project/Area Number 09671881
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionAichi-Gakuin University School of Dentistry

Principal Investigator

IKEDA Takeshi  Aichi-Gakuin Univ.School of Dent. Dept Microbiol.Lecturer, 歯学部, 講師 (80241131)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsPorphyromonas endodontalis / black-pigmentation / vancomycin / antibiotic resistance / 2-D gel electrophoresis / endodontitis / mutant / Porphyromonas gingivalis / クローニング
Research Abstract

Porphyromonas endodontalis, a Gram-negative, black-pigmented, anaerobic rod, is considered to be associated with endodontitis. A nonpigmentd variant, KSEW01, from KSE105 which is a clinical isolate from an endodontic lesion and is possibly a parent strain of KSEW01, was found to be resistant against a glycopeptide antibiotic reagent, or a dalbaheptide, vancomycin (VCM), of which the minimal inhibitory concentration (MIC) was greater than 200 mug/ml. To help understand the mechanism of the antibiotic resistance in anaerobes, the cellular proteins of KSEW01 which was cultured in the presence of and those in the absence of vancomycin were compared by the two dimensonal polyacrylamide gel electrophoresis to detect the component(s) that might give the antibiotic resistance to this strain. A clear spot which was present in the cell cultured with VCM and absent without VCM was found. Regretfully the result was not reproducible. Then, thirty-four VCM-resistant mutants from KSE105, five from P.endodontalis type strain, ATCC35406, were obtained in vitro. MICs of these mutants were measured by agar dilution method. The mutants obtained were all black-pigmented. Some of the mutants had the resistance against VCM to the same degree (MIC, 200 mug/ml) as that of KSEW01. All the three black-pigmented, spontaneous revertants of KSEW01 were isolated and they were also resistant against VCM (MIC, 100 mug/ml). From these results, pigmentation and VCM resistance were not linked together. The detection of the difference between the cellar components of KSE105 and those of the VCM-resistant mutants are under investigation. The results will be applied to a potent pathogen of adult periodontitis, Porphyromonas gingivalis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K.Suzuki, T.Ikeda, H.Nakamura, F.Yoshimura: "Isolation and characterization of a non-pigmented variant of Porphyromonas endodontalis." Oral Microbiol Immunol. 12. 155-161 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Suzuki, T.Ikeda, T.Noguchi, F.Yoshimura: "Detection and prevalence of IS1126, an insertion sequence, in Porphyromonas gingivalis by Southern blot analysis." Dentistry in Japan. 33. 111-115 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Watanabe-Kato, et al.: "Isolation and characterization of transposon-induced mutants of Porphyromonas gingivalis deficient in fimbriation." Microbial Pathogenesis. 24. 25-35 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Suzuki, T.Ikeda, H.Nakamura, F.Yoshimura: "Isolation and characterization of a nonpigmented variant of Porphyromonas endodontalis." Oral Microbiol Immunol. 12. 155-161 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Suzuki, T.Ikeda, T.Noguchi, F.Yoshimura: "Detection and prevalence of IS1126, an insertion sequence, in Porphyromonas gingivalis by Southern blot analysis." Dentistry in Japan. 33. 111-115 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Watanabe-Kato, J.Hayashi, Y.Terazawa, C.I.Hoover, K.Nakayama, E.Hibi, N.Kawakami, T.Ikeda, H.Nakamura, T.Noguchi, F.Yoshimura.: "Isolation and characterization of transposon-induced mutants of Porphyromonas gingivalis deficient in fimbriation." Microbial Pathogenesis. 24. 25-35 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Suzuki, T.Ikeda, H.Nakamura, F.Yoshimura: "Isolation and characterization of a non-pigmented variant of Porphyromonas endodontalis." Oral Microbiol Immunol. 12. 155-161 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Suzuki, T.Ikeda, T.Noguchi, F.Yoshimura: "Detection and prevalence of IS 1126, an insertion sequence, in Porphyromonas gingivalis by Southern blot analysis." Dentistry in Japan. 33. 111-115 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Watanabe-Kato, et al.: "Isolation and characterization of transposon-induced mutants of Porphyromonas gingivalis deficient in fimbriation." Microbial Pathogenesis. 24. 25-35 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Suzuki,T.Ikeda,H.Nakamura,F.Yoshimura: "Isolation and characterization of a non-pigmented variant of Porphyromonas endodontalis." Oral Microbiol Immunol. 12. 155-161 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Suzuki,T.Ikeda,T.Noguchi,F.Yoshimura: "Detection and prevalence of IS 1126,an insertion sequence,in Porphyromonas gingivalis by Southern blot analysis." Dentistry in Japan. 33. 111-115 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Watanabe-Kato,et al.: "Isolation and characterization of transposon-induced mutants of Porphyromonas gingivalis deficient in fimbriation." Microbial Pathogenesis. (印刷中).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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