Effects of TGF-alpha, Trp-P-2 and MeIQx on the Tooth-germ in Organ Culture

• Project/Area Number: 09671882
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Aichi-Gakuin University School of Dentistry
• Principal Investigator: KAMEYAMA Yoichiro Aichi-Gakuin University, School of Dentistry, Department of Pathology, Professor, 歯学部, 教授 (70113066)
• Co-Investigator(Kenkyū-buntansha): MAEDA Hatsuhiko Aichi-Gakuin University, School of Dentistry, Department of Pathology, Associate, 歯学部, 助教授 (30175591)
• Project Period (FY): 1997 – 1998
• Project Status: Completed (Fiscal Year 1998)
• Budget Amount: ¥3,100,000 (Direct Cost: ¥3,100,000); Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: tooth germ / organ culture / growth factor / heterocyclic amine / ヘテロサイクリックアミン

Research Abstract

The purpose of the present study was to investigate histologically and immunohistochemically (BrdU) the effects of growth factor TGF- alpha and carcinogenic heterocyclic amine Trp-P-2 and MelQx on the mouse tooth-germ in organ culture. The mouse tooth-germs were divided into the following 3 experiments (I, II, III) with 7 groups. Experiment I.Group I-A (BGJb) and Group I-B (TGF- alpha). Experiment II.Group II-A (DMSO), Group II-B (Trp-P-2) and Group II-C (MeIQx). Experiment III.Group III-A(TGF- alpha+Trp-P-2) and Group III-B(TGF- alpha+MeIQx).
The results of the present study indicated that TGF- alpha promoted markedly the proliferation of epithelial cells of the mouse tooth-germ , but also suggested that TGF- alpha did not stimulate the differentiation of these epithelial cells. Moreover, it was also suggested that Trp-P-2 and MeIQx suppressed the proliferation of these epithelial cells. It seems from the results of the present study that the development and growth of odontogenic tumors and cysts in humans might be related considerably to the action of TGF- alpha.

Research Products

• [Publications] Fujita, K., Kameyama, Y., Maeda, H., Tanaka, H., Konishi, S., Kato, H and Sato, E.: "Immunohisto-chemical and ultrastructural studies of experimental epithelial dysplasia induced by excisional wounding and trp-P-2 Application in hamster tongues." Oral Med Pathol. 2. 75-80 (1997)
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