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Regulation of beta-agonist-induced secretion of amylase from rat parotid acini by K^+-channels

Research Project

Project/Area Number 09671891
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

MAEDA Sadaaki  Osaka University Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (00135732)

Co-Investigator(Kenkyū-buntansha) MURAYAMA Takafumi  Osaka University Dental Hospital, Resident, 歯学部・附属病院, 医員
SAEKI Makio  Osaka University Faculty of Dentistry, Research Assistant, 歯学部, 助手 (30273692)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsamylase / potassium Channels / parotid / b-agonistsv / phosphorylation
Research Abstract

The involvement of K^+ channels in regulating secretion of amylase from isolated rat parotid acini was studied in conjunction with beta-adrenoceptor function.It was observed that increasing the concentration of extracellular K^+ or adding K^+ channel blockers enhanced the secretion of amylase.Among several K^+ channel blockers, tetraethylammonium, apamin and charybdotoxin were effective to enhance secretion by 48, 69, and 84%, respectively.Glibenclamide was without effect.A low concentration of noradrenaline (10^<-7> M) enhanced secretion by 154% and its simultaneous application with tetraethylammonium gave a synergistic effect, producing 371% stimulation.Combination of tetraethylammonium and a low concentration of carbachol (10^<-6> M) did not give the synergistic effect.Noradrenaline at the concentration of 10^<-6> M enhanced secretion by 313% and this was reduced to 116% by 10^<-3> M valinomycin, a K^+ ionophore.Valinomycin was without effect on carbachol (10^<-5> M)-induced secretion.Somatostatin (10^<-5> M) and morphine (10^<-4> M) also reduced noradrenaline-induced scretion of aimylase.These results suggested the regulation of Ca^<2+> -activated K^+ channels by noradrenaline in amylase secretory processes in parotid acini.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] "Effects of K^+ channel blockers and K^+ ionophoreon is op renaline-induced secretion of amylase from rat parotid acini." European Journal of Pharmacology. 322. 21-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Murayama et al.: "Effect of K+ channel blockers and K+ ionophore on isoprenaline-induced secretion of amylase from rat parotid acini" European Journal of Pharmacol. 322. 21-25 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Murayama et al.: "Effects of K^+ channel blockers and K^+ ionophore on is opren aline-induced secretion of amylase from rat parotid acini." European Journal of Pharmacology. 322. 21-25 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Murayama et al.: "Effects of K^+ channel blockers and K^+ ionophore on isoprenaline-induced secretion of amylase from rat paro tid acini." European Journal of Pharmacology. 322. 21-25 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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