Regulation of beta-agonist-induced secretion of amylase from rat parotid acini by K^+-channels
| Project/Area Number |
09671891
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
MAEDA Sadaaki Osaka University Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (00135732)
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| Co-Investigator(Kenkyū-buntansha) |
MURAYAMA Takafumi Osaka University Dental Hospital, Resident, 歯学部・附属病院, 医員
SAEKI Makio Osaka University Faculty of Dentistry, Research Assistant, 歯学部, 助手 (30273692)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | amylase / potassium Channels / parotid / b-agonistsv / phosphorylation |
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| Research Abstract |
The involvement of K^+ channels in regulating secretion of amylase from isolated rat parotid acini was studied in conjunction with beta-adrenoceptor function.It was observed that increasing the concentration of extracellular K^+ or adding K^+ channel blockers enhanced the secretion of amylase.Among several K^+ channel blockers, tetraethylammonium, apamin and charybdotoxin were effective to enhance secretion by 48, 69, and 84%, respectively.Glibenclamide was without effect.A low concentration of noradrenaline (10^<-7> M) enhanced secretion by 154% and its simultaneous application with tetraethylammonium gave a synergistic effect, producing 371% stimulation.Combination of tetraethylammonium and a low concentration of carbachol (10^<-6> M) did not give the synergistic effect.Noradrenaline at the concentration of 10^<-6> M enhanced secretion by 313% and this was reduced to 116% by 10^<-3> M valinomycin, a K^+ ionophore.Valinomycin was without effect on carbachol (10^<-5> M)-induced secretion.Somatostatin (10^<-5> M) and morphine (10^<-4> M) also reduced noradrenaline-induced scretion of aimylase.These results suggested the regulation of Ca^<2+> -activated K^+ channels by noradrenaline in amylase secretory processes in parotid acini.
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Report
(3 results)
Research Products
(4 results)
