| Project/Area Number |
09671903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido, School of Dentistry |
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Principal Investigator |
TOJYO Yosuke Health Sciences University of Hokkaido, School of Dentistry, Associate Professor, 歯学部, 助教授 (90111731)
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| Co-Investigator(Kenkyū-buntansha) |
NEZU Akihiro Health Sciences University of Hokkaido, School of Dentistry, Instructor, 歯学部, 助手 (00305913)
TANIMURA Akihiko Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor, 歯学部, 講師 (70217149)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Parotid cells / Acinar cells / Intracellular Ca^<2+> / Calcium signal / Inositol-1,4,5-trisphosphate / Intracellular Ca^<2+> store / Ca^<2+> imaging / Ca^<2+>ウェーブ / イノシトール1,4,5-三リン酸 / イソプロテレノール / β受容体 / イメージング / カルシウムウェーブ / リアノジン受容体 |
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| Research Abstract |
1. Imaging of Ca^<2+> waves induced by muscarinic receptor stimulation. The fura-2 loaded rat parotid acinar cells were stimulated with calbachol (CCh). The increase in was initiated in the apical pole of the acinar cells and then rapidly spread as a Ca^<2+> wave towards the basolateral region.
2. The Ca^<2+> wave in saponin-permeabilized parotid acinar cells evoked by flash photolysis of caged 1P3. The permeabilized acinar cells were labelled with the membrane-bound Ca^<2+> indicator Calcium Green C_<18> and stimulated by the photolysis of caged IP The Ca^<2+> release from intracellular stores started at the apical region and spread towards the basolateral region.
3. Imaging of C^<2+> release from intracellular Ca^<2+> stores in permeabilized parotid acinar cells. The Ca^<2+> release from intracellular Ca^<2+> stores was monitored using the fluorescent indicators Mag-fura-2 and Calcium Green C_<18> App1ication of IP_3 caused a rapid release of Ca^<2+>, but none of cyclic ADP-ribose, caffeine or ryanodine stimulated Ca^<2+> release.
4. Effect of isoproterenol on cytosolic Ca^<2+> distribution. Application of 1 muMisoproterenol, a concentration producing the maximum amylase release, did not cause any change in cytosolic Ca^<2+> distribution.
5. Effect of CCh on cytosolic CI distribution. CCh caused a rapid dcrease in cytosolic CF concentration. This change was corelated with the increase in [Ca^<2+>].
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