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Mechanism of osteoclastogenesis inhibitory action by interleukin 18

Research Project

Project/Area Number 09671905
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

UDAGAWA Nobuyuki (1998)  Showa University, School of Dentistry, Lecturer, 歯学部, 講師 (70245801)

宇田川 信之 (1997)  昭和大学, 歯学部, 講師 (20138382)

Co-Investigator(Kenkyū-buntansha) JIMI Eijiro  Showa University, School of Dentistry, Assistant, 歯学部, 助手 (40276598)
TAKAHASHI Naoyuki  Showa University, School of Dentistry, Associate Professor, 歯学部, 助教授 (90119222)
SUDA Tatsuo  Showa University, School of Dentistry, Professor, 歯学部, 教授 (90014034)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsOsteoclast / Osteoblast / Bone resorption / Interleukin 18 / Interferon gamma / GM-CSF / T-lymphocyte / Gene targetting mouse
Research Abstract

IL-18 inhibits osteoclast (CCL) formation in vitro independent of IFN-gamma production, and this was abolished by the addition of neutralizing antibodies to GM-CSF.We now establish that IL-18 was unable to inhibit CCL formation in cocultures using GM-CSF-deficient mice (GM-CSF-/-). Reciprocal cocultures using either wild-type osteoblasts with GM-CSF -/- spleen cells or GM-CSF -/- osteoblasts with wild-type spleen cells were examined. Wild-type spleen cells were required to elicit a response to IL-18 indicating that cells of splenic origin were the IL-18 target. As T cells comprise a large proportion of the spleen cell population, the role of T cells in osteoclastogenesis was examined. Total T cells were removed and repleted in various combinations. Addition of wild-type T cells to a GM-CSF -/- coculture restored IL-18 inhibition of osteoclastogenesis. Major subsets of T cells, 0D4 and CD8+^+, were also individually depleted. Addition of either CD4^+ or CD8^+ wild-type T cells restored IL-18 action in a GM-CSF -/- background, while IL-18 was ineffective when either CD4^+ or CD8^+ GM-CSF -/- T cells were added to a wild-type coculture. These results highlight the involvement of T cells in IL-18-induced CCL inhibition and provide evidence for a new CCL inhibitory pathway whereby IL-18 inhibits CCL formation due to action upon T cells promoting the release of GM-CSF, which in turn acts upon CCL precursors.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Suda, T., et al.: "Modulation of osteoclast differentiation and function by the new members of the TNF receptor and ligand families." Endocrine Reviews. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kwon, B.S., et al.: "A new member of tumor necrosis factor receptor superfamily TR1, induces fibroblast proliferation and inhibits osteoclastogenesis and bone resorption." FASEB J.12. 845-854 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuzaki, K., et al.: "Osteoclast differentiation factor(ODF)induces osteoclast-like cell formation in human peripheral blood mononuclear cell cultures." Biochem.Biophys.Res.Commun.246. 199-204 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuda, H., et al.: "Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and identical to TRANCE/RANKL." Proc.Natl.Acad.Sci.USA. 95. 3597-3602 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Horwood, N.J., et al.: "Interleukin-18 inhibits osteoclast formation via T-cell production of granulocyte/macrophage colony-stimulating factor." J.Clin.Invest.101. 595-603 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Udagawa, N., et al.: "Interleukin-18(interfero-γ-inducing factor)is produced by osteoblasts and acts via granulocyte/macrophage-colony stimulating factor and not via interferon-γ to inhibit osteoclast formation." J.Exp.Med.185. 1005-1012 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suda, T.et al.: "Modulation of osteoclast differentiation and function by the new members of the TNF receptor and ligand families." Endocrine Reviews. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kwon, B.S.et al.: "A new member of tumor necrosis factor receptor superfamily TR1, induces fibroblast proliferation and inhibits osteoclastogenesis and bone resorption." FASEB J.12. 845-854 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuzaki, K.et al.: "Osteoclast differentiation factor (ODF) induces osteoclast-like cell formation in human peripheral blood mononuclear cell cultures." Biochem.Biophys.Res.Commun.246. 199-204 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuda, H.et al.: "Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and identical to TRANCE/RANKL." Proc.Natl.Acad.Sci.USA. 95. 3597-3602 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Horwood, N.J.et al.: "Interleukin-18 inhibits osteoclast formation via T-cell production of granulocyte/macrophage colony-stimulating factor." J.Clin.Invest. 101. 595-603 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Udagawa, N.et al.: "Interleukin-18 (interfero-gamma-inducing factor) is produced by ostoblasts and acts via granulocyte/macrophage-colony stimulating factor and not via interferon-gamma to inhibit osteoclast formation." J.Exp.Med.185. 1005-1012 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suda,T.,et al.: "Modulation of osteoclast differentiation and function by the new members of the TNF receptor and ligand families." Endocrine Reviews,in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsuzaki,K.,et al.: "Human osteoclast-like cells are formed from peripheral blood mononuclear cells in a co-culture with SaOS-2 cells transected with the PTH/PTHrP receptor gene." Endocrinology. 140. 925-932 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsukii,K.,et al.: "Osteoclast differentiation factor mediates an essential signal for bone resorption induced by 1α,25-dihydroxyvitamin D_3,prostaglandin E_2 or parathyroid hormone in the microenvironment of bone." Biochem.Biophys.Res.Commun.246. 337-341 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katayama,Y.,et al.: "Casein kinase 2 phosphorylation of recombinant rat osteopontin enhances adhesion of osteoclasts but not osteoblasts." J.Cell.Physiol.176. 179-187 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kwon,B.S.,et al.: "A new member of tumor necrosis factor receptor superfamily TR1,induces fibroblast proliferation and inhibits osteoclastogenesis and bone resorption." FASEB J.12. 845-854 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsurukai,T.,et al.: "Isolation and characterization of osteoclast precursors that differentiate into osteoclasts on calvarial cells within a short period of time." J.Cell.Physiol.177. 26-35 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Udagawa,N.,et al.: "Interleukin-18 (interferon γ-inducing factor) is produced by osteoblasts and acts via granulocyte/macrophage-colony stimulating factor and not via interferon-γ to inhibit osteoclast formation." J.Exp.Med.185. 1005-1012 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wada,S.,et al.: "Regulation by calcitonin and glucocorticoids of calcitonin receptor gene expression in mouse osteoclasts." Endocrinology. 138. 521-529 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakamura,I.,et al.: "Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice." FEBS Lett.401. 207-212 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murakami,H.,et al.: "Tiludronate inhibits protein tyrosine phosphatase activity in osteoclasts." Bone. 20. 399-404 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Amizuka,N.,et al.: "An ultrastructural study of cell-cell contact between mouse spleen cells and calvariaderived osteoblastic cells in a co-culture system for osteoclast formation." Act.Histochem.Cytochem.30. 351-362 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Horwood,N.J.,et al.: "Interleukin-18 inhibits osteoclast formation via T-cell production of granulocyte/macrophage colony-stimulating factor." J.Clin.Invest.101. 595-603 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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