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BIOGENESIS OF SECRETORY GRANULES IN PAROTID ACINAR CELLS

Research Project

Project/Area Number 09671909
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionNIHON UNIVERSITY

Principal Investigator

YOKOYAMA Miki  NIHON UNIVERSITY SCHOOL OF DENTISTRY AT MATSUDO LECTURER, 松戸歯学部, 講師 (70191533)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsRAT PAROTID / SMALL-MOLECULAR-MASS GTP-BINDING PROTEINS / ACINAR CELLS / SECRETORY GRANULES / EXOCRINE GLANDS / SALIVERY GLANDS / ADP-RIBOSYLATION FACTOR / ADPリボシレーション因子
Research Abstract

In the previous study (1997), we demonstrated that ADP-ribosylation factor (Arf) is present in the cytosolic fraction of rat parotid acinar cells and Arf translocates to the secretory granules in a GTp-dependent manner. So far, six mammalian Arf genes (Arf1-Arf6) have been reported. In bovine adrenal chromaffin cells, Arf6 is associated to the secretory granules. Now we have succeeded in demonstrating the following two points to elucidate the role of Arf proteins on the secretory granules. These results were published in Archives of Biochemistry and Biophysics 357, 147-154 (1998).
1. Identification of the Arf isotype as Arfi1that translocates to the secretory granules
We identified the Arf isotype that translocates to the secretory granules by using antibodiesspecific for Arf1, Arf3, Arf5, and Arf6. In contrast to the Arf6 localization to the secretory granules in bovine adrenal chromaffin cells, Arf6 was not detected in the granule membrane fraction of rat parotid acinar cells.
2. Demonstration of the Arfi translocation to the secretory granules by immunoelectron microscopy
To confirm directly that Arf1 is translocated to the intact secretory granules, the secretorygranules were isolated after the translocation experiment and used for the immunoelectron microscopy with anti-Arf antibody. Gold particles were detected around the surface of the mature secretory granules, about 1 mum in diameter.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Yoko Dohke et al.: "ADP-RIBOSYLATION FACTORS IN RATPAROTID ACINAR CELLS" European Journal of Morphology. 36. 186-189 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoko Dohke et al.: "Translocation of Arfl to the Secretory Granules in Rat Parotid Acinar Cells" ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 357(1). 147-154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Junko Fujita-Yoshigaki et al.: "SNARE PROTEINS FOR CYCLIC AMP-REGULATED EXOCYTOSIS IN SALIVARY GLANDS" European Journal of Morphology. 36. 46-49 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiromi Michikawa et al.: "cGMP production is coupled to Ca^<2+>-dependent nitric oxide generation in rabbit parotid acinar cells" Cell Calcium. 23(6). 405-412 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Dohke, M.Hara-Yokoyama, J.Fujita-Yoshigaki, R.A.Kahn, Y.Kanaho, S.Hashimoto, S.Furuyama and H.Sugiya: "Translocation of Arf1 to the Secretory Granules in Rat Parotid Acinar Cells" Archives of Biochemistry and Biophysics. 357(1). 147-154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Dohke, M.Hara-Yokoyama, J.Fujita-Yoshigaki, S.Furuyama and H.Sugiya: "ADP-RIBOSYLATION FACTORS IN RAT PAROTID ACINAR CELLS" European Journal of Morphology. 36. 186-189 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] J.Fujita-Yoshigaki, Y.Dohke, M.Hara-Yokoyama, S.Furuyama and H.Sugiya: "SNARE PROTEINS ESSENTIAL FOR CYCLIC AMP-REGULATED EXOCYTOSIS IN SALIVARY GLANDS" European Journal of Morphology. 36. 46-49 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Michikawa, Y.Mitsui, J.Fujita-Yoshigaki, M.Hara-Yokoyama, S.Furuyama and H.Sugiya: "cGMP production is coupled to Ca^<2+> -dependent nitric oxide generation in rabbit parotid acinar cells" cell Calcium. 23(6). 405-412 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoko Dohke et al.: "ADP-RIBOSYLATION FACTORS IN RAT PAROTID ACINAR CELLS" European Journal of Morphology. 36. 186-189 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoko Dohke et al.: "Translocation of Arfl to the Secretory Granules in Rat Parotid Cells" ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. 357(1). 147-154 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Junko Fujita-Yoshigaki et al.: "SNARE PROTEINS FOR CYCLIC AMP-REGULATED EXOCYTOSIS IN SALIVARY GLANDS" European Journal of Morphology. 36. 46-49 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiromi Michikawa et al.: "cGMP production is coupled to Ca^<2+>-dependent nitric oxide generation in rabbit parotid acinar cells" Cell Calcium. 23(6). 405-412 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Dohke: "ADP-RIBOSYLATION FACTORS IN RAT PAROTID ACINAR CELLS" European Journal of Morphology. (印刷中).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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