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Expression and function of HGF in salivary gland

Research Project

Project/Area Number 09671913
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka Dental University

Principal Investigator

KAMADA Aiko  Osaka Dental University, School of Dentistry, Research Assistant, 歯学部, 助手 (50140215)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsSubmandibular Gland / HGF / c-Met / RT-PCR / Heparan Sulfate / Proteoglycan
Research Abstract

I investigated the characteristics and mRNA expression of hepatocyte growth factor (HGF) in the adult female monkey submandibular gland. HGF was extracted with PBS containing protease inhibitors, and purified partially by heparin affinity chromatography. With Western blot analysis, the HGF fraction showed an immunopositive protein band of 69 kDa corresponding to the a-subunit of HGF.The gene expression for HGF and its high affinity receptor, c-Met, were revealed in the monkey submandibular gland using RT-PCR, and the PCR products showed high homology to cDNA for human. Furthermore, in situ RT-PCR technique demonstrated that both the mRNA signal for HGF and c-Met were localized exclusively to the striated and excretory ductal cells. Hence, these results indicate that both HGF and c-Met are synthesized in the same cells in monkey submandibular glands.
Moreover, I investigated the characteristics and he mRNA expression of monkey submandibular gland heparan sulfate proteoglycan (HSPG), which is a low affinity receptor of HGF.PBS-soluble HSPG and microsomal membrane HSPG were extracted from submandibular glands, and purified partially by ion exchange chromatography. Western blot analysis demonstrated several PBS-soluble HSPGs and only a microsomal membrane HSPG.The RT-PCR technique revealed the mRNA expression of syndecan-1, syndecan-2, syndecan-4 and glypican-1, which are cell-surface HSPGs. These results indicated that the cell-surface HSPGs are synthesized in the monkey submandibular glands, and that their ectodomains are released into the extracellular matrix.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] T Moroto, A Kamada: "Expression and localization of HGF mRNA in monkey submandibular gland" Journal of Osaka Dental University. 32・2. 37-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A Kamada et al.: "Characteristics nad mRNA expression of monkey submandibular glad proteoglycans" Journal of Dental Research. 77・. 727- (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Moroto and A Kamada: "Expression and localization of HGF mRNA in monkey submandibular gland" J Osaka Dent Univ. 32(2). 37-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A Kamada et al: "Characteristics and mRMA expression of monkey submandibular gland proteoglycans" J Dental Res. 77. 727 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Moroto,A Kamada: "Expression and localization of HGF mRNA in monkey submandibular gland" Journal of Osaka Dental University. 32・2. 37-42 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A Kamada et al.: "Characteristics and mRNA expression of monkey submandibular gland proteoglycans" Journal of Dental Research. 77・. 727 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鎌田愛子、他: "サル顎下腺HGFの生化学的性状" 歯科基礎医学会雑誌. 39・補冊. 488 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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