Inhibitory effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins derived from Streptococcus mutans on the induction of dental caries
| Project/Area Number |
09671926
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
病態科学系歯学(含放射線系歯学)
|
|---|
| Research Institution | KYUSHU UNIVERSITY |
|---|
Principal Investigator |
OHO Takahiko Faculty of Dentistry, KYUSHU UNIVERSITY Assistant Professor, 歯学部, 講師 (50160940)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Dental Caries / Streptococcus mutans / Adhesion Factor / Fusion Protein / Protein Antigen / Glucosyltransferase / Dental Caries Prevention |
|---|
| Research Abstract |
Cell surface protein antigen (PAc) and glucosyltransferases (GTFs) produced by Streptococcus mutans are considered to be major colonization factors of the organism.
In this study, fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region (PAcA) of PAc with the glucan binding (GB) domain of GTF-I, an enzyme catalyzing the synthesis of water-insoluble glucan from sucrose, and fusion protein PAcA-SB, a fusion of PAcA with the sucrose binding (SB) domain of GTE-I were constructed. The recombinant fusion proteins were purified, and rabbit antibodies against these fusion proteins were prepared. Water-insoluble glucan synthesis by cell-associated and cell- free GTF preparations from S.mutans as well as total glucan synthesis by GTF-I was markedly inhibited by anti-PAcA-GB antibodies but not by anti- PAcA-SB antibodies. Significant inhibition of the sucrose-independent and sucrose-dependent adhesion of S.mutans to saliva-coated hydroxyapatite beads was observed when anti-PAcA-GB antibodies were added to the reaction mixture. Anti-PAcA-SB antibodies inhibited the adhesion of S.mutans to the beads in the absence of sucrose but not in the presence of sucrose. immunization with the fusion protein PAcA-GB may be useful for controlling the colonization of teeth by S.mutans.
|
Report
(3 results)
Research Products
(18 results)
