| Project/Area Number |
09671934
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Nihon University |
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Principal Investigator |
KOMIYAMA Kazuo Nihon University.School of Dentistry, Associate professor, 歯学部, 助教授 (00120452)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | agammaglobulinemia / aly / aly mouse / IL-2 / Cytokine / RT-PCR / IgA deficient / Iymphocyte / Secretory component / AGAMMAGLOBULINEMIA / Cytokine / Lymphocyte / Secretory component / hypogammaglobulinemia |
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| Research Abstract |
Agaminaglobulinemia is serve immunodeficient disease that resulted in a lack of particular immunoglobulins. In current study, we have examined T cell function of aly/aly mice as a model of agammaglobulinemia that the mice revealed a severe combined immunodificiency including a lack of IgA and IgG synthesis. The aly/aly mice are also a lack of Peyer's patches. On the histological examination, the mice revealed unclear margin of medulla and cortex in thymus. The number of follicles in the spleen was reduced in compared with conventional mice. The lymphocytes were isolated from spleen and measured T/B cell ratio by FACS.Aly/aly mice showed normal T/B cell ratios however the total recovered cell numbers were clearly low than conventional mice. In vivo and In vitro antigen stimulation of the aly/aly mice revealed no antibody response. The cytokine profiles of T cells were examined by RT-PCR.We found that IL-2 mRNA synthesis was quite low revels in the aly/aly mice, while other cytokines mRNA syntheses were constantly detected by RT-PCR.The IL-2 receptor expression in T cell was also in low levels. This IL2 deficiency was proved by an anti-CD3 treated cell proliferation assay, ELISA and FACS analysis. The size and number of colony formation after CD3 and PMA stimulation of T cells were decreased. IgM antibody response was recovered after IL2 adding to in vitro antigen stimulation assay. An experiment of mix lymphocyte culture revealed low levels of B cell response. The establishment of T cell clone was not succeeded. The result indicated that agammaglobulinemia of the aly/aly mice may be caused by the deficient of IL2 synthesis in T cells. However, B cell function was also involved in this immunodeficiency. Farther molecular base analysis was required for resolve T cell dysfunction in an agammaglobulinemia.
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