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Study on the Iron-Acquisition Mechanism of Periodontal Bacteria

Research Project

Project/Area Number 09671953
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionOkayama University

Principal Investigator

KOKEGUCHI Susumu  Okayama Univ.Dent.Sch., Assistant, 歯学部, 助手 (10144776)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Keiso  Okayama Univ.Dent.Sch., Assistant, 歯学部, 助手 (70243475)
NISHIMURA Fusanori  Okayama Univ.Dent.Sch., Associate Professor, 歯学部附属病院, 講師 (80208222)
MURAYAMA Yoji  Okayama Univ.Dent.Sch., Professor, 歯学部, 教授 (50029972)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsIron / Acquisition / Periodontal Bacteria / Porphyromonas gingivalis / Actinobacillus actinomycetemcomitans
Research Abstract

Iron, an essential bacterial nutrient, is not freely available for bacteria in vivo because iron is complexed with host iron-binding proteins such as transferrin, lactoferrin and hemoglobin. However, pathogenic bacteria develop specific iron-uptake systems including the production of specific cellular iron binding proteins to acquire iron in vivo. For periodontal bacteria, little is known about their iron-uptake system. In this study, we determined the genes for iron uptake in periodontal bacteria, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The following results were obtained.
1) A.actinomycetemcomitans :
The two ferritin genes were found in the upstream of a regulatory gene, actX of A.actinomycetemcomitans Y4. Ferritin is a bacterial iron binding protein, which acts iron acquisition and storage in bacterial cells. The ferritin genes in A.actinomycetemcomitans Y4 were cloned and their DNA sequence analyses were performed. They are consisted of 486 bp (161 amino ac … More ids) and 498 bp(165amino acids) open reading frames. They exhibited 80 % and 85 % homology with Haemophilus influenzae ferritin genes. The Western blotting analysis showed the two cellular proteins(approximately 20kDa), reacted with anti-Escherichia coli ferritin rabbit serum were expressed in A.actinomycetemcomitans Y4 cells.
2) P.gingivalis :
P.gingivalis requires hemin for its growth. The putative hemin biding protein genes were determined for P.gingi va/is by polymerase chain reaction method. The oligonucleotide primers for the PCR were designed from the Bacteroides fragilis hemin-uptake protein gene(hupA). Approximate 1.3-kb DNA fragments were amplified from P.gingivahs FDC 381 genomic DNA and the DNA sequences were analyzed. The partial DNA sequences of the amplified DNA fragments showed high homologies with the P.gingi valis hemagglutinin gene and protease genes. These results indicate that P.gingivalis might capture to erythrocytes and decompose them to acquire hemin by these gene products. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Hongyo H., Kurihara H., Kokaguchi S., et al.: "Molecular cloning and characterization of the gene encoding 53kD outer membrane protein of Porphyromonas gingivalis" Microbios. 92. 47-52 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyamoto M., Maeda H., Kitanaka M., Kokeguchi S., et al.: "The S-layer protein from campylobactor rectus : sequence determination and function of the recombinant protein" FEMS Microbiology Letters. 166. 275-281 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hongyo H., Kokeguchi S., Kurihara H., et al.: "Comparative study of two outer membrane protein genes from Porphyromonas gingivalis" Microbios. 95. 91-100 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 澤田聡子, 苔口進, 宮本学 等: "細菌16SリボソームRNA遺伝子に基づくDNAプローブを用いた歯周病細菌の同定 -コロニーハイブリダイゼーション法-" 日本歯周病学会会誌. 40(4). 475-485 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohyama H., Matsushita S., Kato N., Nishimura F., et al.: "T Cell Responses to 53-kDa Outer Membrane Protein of Porphyromonas gingivalis in Human with Early-Onset Periodontitis" Human Immunology. 59. 635-643 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kokeguchi S., Miyamoto M., Maeda H., et al.: "Characteristics of outer membrane protein of Actinobacillus actinomycetemcomitans grown with malfose" Journal of Dental Research. 41. 59 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hongyo, H., Kurihara, H., Kokeguchi, S., et al.: "Molecular cloning and characterization of the gene encoding 53kD outer membrane protein of Porphyromonas gingivalis." Microbios. vol.92. 47-52 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyamoto, M., Meada, H., Kitanaka, M., Kokeguchi, S., et al.: "The S-layer protein from Campylobacter rectus. Sequence determination and function of the recombinant protein." FEMS Microbiol.Lett.vol.166. 275-281 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hongyo, H., Kokeguchi, S., H., Kurihara, H., et al.: "Comparative study of two outer membrane protein genes from Porphyromonas gingivalis." Microbios. vol.95. 91-100 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohyama, H., Matsushita, S., Kato, N., Nishimura, F., Oyaizu, K., Kokeguchi, S., et al.: "T Cell Responses to 53-kDa Outer Membrane Protein of Porphyromonas gingivalis in Humans with Early-Onset Periodontitis." Human Immunol.vol.59. 635-643 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kokeguchi, S., Miyamoto, M., Maeda, H., et al.: "Characteristics of Outer Membrane Protein of Actinobacillus actinomycetemcomitans Grown with Maltose." J.Dent.Res.vol.77. 985 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hongyo H., Kurihara H., Kokeguchi S., et al.: "Molecular cloning and characterization of the gene encoding 53KD outer membrane protein of Porphyromonas gingivalis" Microbios. 92. 47-52 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyamoto M., Maeda H., Kitanaka M., Kokeguchi S., et al.: "The S-layer protein from Campylobcter rectus : sequence determination and function of the recombinant protein" FEMS Microbiology Letters. 166. 275-281 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hongyo H., Kokeguchi S., Kurihara H., et al.: "Comparative study of two outer membrane protein genes from Porphyromonas gingivalis" Microbios. 95. 91-100 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 澤田聡子、苔口進、宮本学 等: "細菌16Sリボソ-αRNA遺伝子に基づくDNAプローブを用いた歯周病細菌の同定-コロニーハイブリダイゼーション法-" 日本歯周病学会会誌. 40(4). 475-485 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kokeguchi S., Miyamoto M., Maeda H., et al.: "Characteristics of Outer mumbrane Protein of Actinobacillus actinomycetemcomitans Grown with Maltose" Journal of Dental Research. 77. 985 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 弘末勝、苔口進、澤田弘一 等: "Actinobacillus actinomycetemcomitansの病原性関連遺伝子群" 日本歯科保存学雑誌. 41. 59 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hongyo,H., Kurihara,H., Kokeguchi,S., et al.: "Molecular cloning and characterization of the gene encoding 53-KD outer membrane protein of Porphyromonas gingivalis." Microbios. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sawada,K., Kokeguchi,S., et al.: "Identification of a novel insertion seguence element from Porphyromonas gingivalio W83." Journal Dental Research. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kokeguchi,S., Miyamoto,M., et al.: "A Regulatory Gene Controlling Growth of Actinobacillus dctinomycetem comitans." Journal Dental Research. 76. 224 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda,H., Kitanaka,M., Kokeguchi,S., et al.: "Molecular and biological features of S-layer protein from Campylobacter rectus." Journal Dental Research. 76. 224 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 澤田弘一, 苔口進, 等他: "Porphyromonas gingivalisの膿瘍形成遺伝子のサブトラクティブハイブリダイゼーション法による研究" 日本歯周病学会会誌. 35. 119 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 澤田聡子, 苔口進, 等他: "16S ribosomal RNA遺伝子を用いる口腔内運動性細菌の系統分類" 日本歯科保存学雑誌. 40. 57 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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