• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of neutrophil apoptosis in periodontitis: possible involvement of Fas/FasL and Fas-dependent signaling pathway

Research Project

Project/Area Number 09671962
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionMeikai University

Principal Investigator

SHIINOJIMA Takahiro  Meikai Univ. Sch. Of Dentistry, Lecturer, 歯学部, 講師 (60146230)

Co-Investigator(Kenkyū-buntansha) TATSUMI Junichi  Meikai Univ. Sch. Of Dentistry, Lecturer, 歯学部, 講師 (60227105)
栗原 徳善  明海大学, 歯学部, 講師 (10186512)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsNeutrophil / Apoptosis / Eas / FasL / sFas / sFasL / Lipopolysaccharide / Periodontal tissue destruction / sFasL
Research Abstract

Many factors, such as cytokines and bacterial products, are known to affect the life span of PMNs. The author investigated whether P.gingivalis LPS retards the apoptotic process of PMNs and, if so, whether the delayed apoptosis is related to Fas, FasL, or soluble form of these molecules. During incubation in the regular culture medium, the viability of PMNs progressively declined, producing apoptotic cells, characterized by chromatin condensation, internucleosomal DNA fragmentation, cell shrinkage and blebbing of plasma membrane. P.gingivalis LPS significantly increased the viability of PMNs and reduced the production of fragmented DNA, as efficiently as E.coli LPS. Western blot analysis demonstrates that Fas expression was not changed but FasL expression slightly declined. sFas and sFasL expression were increased when PMNs were treated with LPS. Among five sFas variants, only Fas Exo6 Del was detectable. Fas mRNA expression was increased whereas there is little difference of FasL mRNA expression. The present study suggests that the retardation of PMNs apoptosis by P.gingivalis LPS may modulate the restoration of acute inflammation in the periodontal tissues, where activated PMNs accumulate. The delayed apoptosis can partly be explained by Fas/FasL system. The author propose here that this inhibition of PMNs apoptosis may be related to increased sFas(Fas Exo6 Del) and processing of membrane-bound FasL by LPS during early stage of apoptosis.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Hiroi M, Shimojima T et al: "Re-evaluation of the culture condition of polymorphonuclear cell for the study of apoptosis induction"Anticancer Res.. 18. 1813-1817 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroi M, Shimojima T et al: "Inhibition by Porphyromanas gingivalis LPS of apoptosis induction in human peripheral blood polymorphonuclear leukocytes."Anticancer Res.. 18. 3475-3480 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroi M, Tajima M, Shimojima T, Kashimata M, Miyata T and Sakagami H: "Re-evaluation of the culture condition of polymorphonuclear cells for the study of apoptosis induction."Anticancer Res. 18. 1813-1817 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroi M, Shimojima T, Kashimata M, Miyata T, Takahama M and Sakagami H: "Inhibition by Porphyromonas gingivalis LPS of apoptosis induction in human peripheral blood polymorphonuclear leukocytes."Anticancer Res.. 18. 3475-3480 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroi M, Shimojima T et al.: "Re-evaluation of the culture condition of polymorphonuclear cells for the study of apoptosis induction"Anticancer Res. 18. 1813-1817 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hiroi M, Shimojima T et al.: "Inhibition by Porphyromonas gingivalis LPS of apoptosis induction in human peripheral blood polymorphonuclear leukocytes"Anticancer Res. 18. 3475-3480 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Hiroi: "Inhibition by Porphyromonas gingivalis LPS of Apoptosis Induction in Human Peripheral Bllod Polymorphonuclear Leukocytes" Anticancer Res.18. 3475-3480 (1998)

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi