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Evaluation of periodontal pathogenic microorganisms and periodontitis using rapid DNA probe method.

Research Project

Project/Area Number 09671965
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

TAKAHASHI Junichi  Tokyo Dental College, Dept. of Dentistry, Assistant, 歯学部, 助手 (30287180)

Co-Investigator(Kenkyū-buntansha) KOMIYA Akiyo  Tokyo Dental College, Dept. of Dentistry, Assistant, 歯学部, 助手 (00307381)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRapid DNA probe / Porphyromonas gingivalis / Actinobacillus actinomycelemcomitans / Periodontal disease-associated bacteria / Rapid diagnosis / Periodontal initial therapy / Porphyromonas gingivalis / Actinobacillus actinomycetemcomitans / 歯周炎 / 診断法
Research Abstract

The aim of the present study was to develop a rapid DNA probe method for the diagnosis of periodontitis that can be used in the dental clinics. The method minimizes the use of water bath for ordinary hybrydization and washing in order to shorten the total time. Samples were hybridized with boiled DNA probe, and then washed thoroughly with 0.2×SSC/0.1% SDS at 75℃. Detection process could be completed within 2 hours. Detection level was more than 10ィイD14ィエD1 bacterial cells/sample.
Subgingival plaque samples were taken from 468 sites in 55 patients with periodontitis before the periodontal initial therapy. After the periodontal therapy of scaling and rootplaning, 58 sites in 9 patients with periodontitis were bacteriologically and cliically evaluated. When the DNA probe method was compared with the culture method, the acuracy was 88% for P. gingivalis, 67% for A. actinomycetemcomitans. A significant association was found between the detection of P. gingivalis and PD, BOP (x 2 test, p<0.01). A significant association was also shown between the detection of A. actinomycetemcomitans and PD in patients whose ages were 35 or older (x 2 test, p<0.01). The relationship between A. actinomycetemcomitans and BOP was not significant. The detection rate of A. actinomycetemcomitans was highest in teenagers. At shallow periodontal pocket sites (PD<4mm) in teenagers, no P. gingivalis was found, while 22% of the sites harbored A. actinomycetemcomitans. After the therapy, the proportions of P. gingivalis decreased significantly only in the clinically resolved sites(% 2 test, p<0.01). However, A. actinomycetemcomitans seemed to persist after the therapy.
The rapid DNA probe method appears promising as an efficient tool for rapid diagnosis, selection of treatment modalities, and microbiological evaluation of the treatment outcome.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Akiyo Komiya: "A rapid DNA robe method for detection of Porrhyromonas gingivalis and Actinobacillus actinomycetemcomitans"Journal of Periodontology. No.3. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小宮明代: "Porphyromons gingivalisとActinobacillus actinomycetemcomitansに対する簡便DNAプローブ法の開発と臨床応用"日本歯周病学会誌. 40巻2号. 162-174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akiyo Komiya: "A rapid DNA probe method for detection of Porrhyromonas gingivalis and Actinobacillus actinomycetemcomitans"Journal of Periodontology. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akiyo Komiya: "Development of a Rapid DNA probe method for Porrhyromonas gingivalis and Actinobacillus actinomycetemcomitans and its clinical application"Journal of Japanese Society of Periodontology. 40(2). 162-174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akiyo Komiya: "A rapid DNA probe method for detection of Porrhyromonas gingivalis and Actinobacillus actinomycetemcomitans"Journal of Periodontology. No.3. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 小宮明代: "Porphyromons gingivalisとActinobacillus actinomycetemcomitansに対する簡便DNAプローブ法の開発と臨床応用"日本歯周病学会誌. 40巻2号. 162-174 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Junichi Takahashi,Atsushi Saito,Taneaki Nakagawa,Satoru Yamada,Kazuyuki Ishihara and Atsugi Okuda: "Dynamics of Serum Immunoglobulin G Avidity for Porphyromonas gingivalis in adult Periodontitis" Journal of Periodontology. 69・3. 367-373 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 高橋潤一: "Porphyromonas gingivalisに対する成人性歯周炎患者血清中のIgGアビディティについて" 日本歯周病学会誌. 38・3. 261-271 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中川種昭,齋藤 淳, 高橋潤一,小宮明代,穂坂康明,山田 了,奥田克爾: "Evalusite Periodontal Testの歯周病関連性細菌の検出能について" 日本歯周病学会誌. 37・2. 312-316 (1995)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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