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Acceleration of bone healing process by local application of the resorbable collagen pellet containing basicfibroblast growth factor (FGF-2)

Research Project

Project/Area Number 09671990
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

HOSOKAWA Ryuji  Hiroshima University, School of Dentistry, Research Associate, 歯学部, 助手 (60211546)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Mitsuyoshi  Hiroshima University, School of Dentistry, Research Associate, 歯学部, 助手 (50284211)
KUBO Takayasu  Hiroshima University, School of Dentistry, Research Associate, 歯学部, 助手 (60240876)
WADAMOTO Masayoshi  Hiroshima University, Dental Hospital, Assistant Professor, 歯学部・附属病院, 講師 (70231040)
AKAGAWA Yasumasa  Hiroshima University, School of Dentistry, Professor, 歯学部, 教授 (00127599)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsFGF-2 / primary culture / bone regeneration / drug delivery system / dog / chondrocytes
Research Abstract

We performed an animal study to ascertain whether the regeneration of membrane-protected bone defects can be accelerated by the controlled application of basic fibroblast growth factor (FGF-2) using a new drug delivery system. First, we investigated in vitro effects of FGF-2 on the mineralization process in primary cultures of dog growth plate chondrocytes. Chondrocytes were isolated from the growth plates of ribs of 1-week-old dogs. The chondrocytes were maintained at extremely high density (5 x 10^4 cells/well) on collagen-coated 96-well dishes in a-MEM with 10% fetal bovine serum and 50 mug/ml ascorbic acid. Mineralization was initiated between days 20 and 24 ; however, the addition of fibroblast growth factor (FGF)-2 (1.0 ng/ml) suppressed mineralization. Second, we performed in vivo study using beagle dogs. Alveolar bone defects were made surgically in 9 beagle dogs, and FGF-2 was administered using specially made collagen pellets. A pellet containing either 0.15 mug FGF-2 (FGF) or 0 mug FGF-2 (placebo) was placed in the defect or no minipellet was used (control), and bone regeneration was evaluated radiologically, histologically, and histomorphometrically 8 weeks after the operation. X-ray radiographs showed a surprisingly large radiopaque region in FGF sites compared with placebo or control sites. Histologically, mature bone filled the majority of the inner space of the membrane-protected defect in FGF sites. New bone formation was also seen in the control and the placebo sites, however, it filled less than half the area of the defect. Histomorphometrically, the area of regenerated bone in FGF sites was significantly higher than in the other sites (p<0.01). These results demonstrate that the controlled application of FGF-2 accelerates bone regeneration in membrane-protected bone defects.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Ryuji Hosokawa et al.: "Controlled local application of basic fibroblast growth factor(FGF-2) accelerates the healing of GBR:An experimental study in beagle dogs" Clinical Oral Implants Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ryuji Hosokawa et al.: "Primary Culture of Canine Growth Plate Chondrocytes as a Model of Biomineralization" Journal of Hard Tissue Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hosokawa R,Kikuzaki K,Kimoto T,Matsuura T,Chiba D,Wadamoto M,Sato Y,Maeda M,Sano A,Akagawa Y :"Controlled local application of basic fibroblast growth factor (FGF-2) accelerates the healing of GBR : An experimental study in beagle dogs" Clinical Oral Implants Research. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ryuji Hosokawa, Kenji Kikuzaki, Daisuke Chiba and Yasumasa Akagawa: "Primary Culture of Canine Growth Plate Chondrocytes as a Model of Biomineralization" Journal of Hard Tissue Biology. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ryuji Hosokawa et al.: "Controlledlocal applicaion of basic fibroblast growth factor (FGF-2) acceleates the healing of GBR : An experimental study in beagle dogs" Clinical Oral Inplants Research.

    • Related Report
      1998 Annual Research Report
  • [Publications] Ryuji Hosokawa et al.: "Primary Culture of Canine Growth Plate Chondrocytes as a Model of Biomineralization" Journal of Hard Tissue Biology.

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimoto,T., et al.: "Continuous administration of basic fibroblast growth factor (FGF-2)accelerates bone induction on rat calvaria" Journal of Dental Research. (in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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