| Project/Area Number |
09671993
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ASAOKA Kenzo The University of Tokushima, School of Dentistry, Professor, 歯学部, 教授 (50014189)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Yukiko The University of Tokushima, School of Dentistry, Research Associate, 歯学部・附属病院, 助手 (60294708)
KON Masayuki The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (80116813)
寺井 邦博 徳島大学, 歯学部, 助手 (10304536)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Calcium phosphate cement / Bone filling material / Root canal filling material / Tricalcium phosphate / Hydroxyapatite / Biocompatibility / Mixing liquid / Mechanical property / 骨補墳材 / 結晶析出 / 骨髄填材 / 生体親和剤 / α型リン酸3カルシウム / 濃度 / Ca / P比 |
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| Research Abstract |
The purpose of this study was to develop an α-tricalcium phosphate (α-TCP) cement which transforms to hydroxyapatite (HAP) in a relatively short period. Calcium and phosphate solutions were used as the liquid phase for the α-TCP cement. The α-TCP powder was first mixed with 1.0 mol/L calcium chloride solution, and then mixed with 0.6 mol/L sodium dihydrogen phosphate solution. X-ray diffraction analysis revealed that the set cement was mostly transformed to HAP within 24h when kept in an incubator. Moreover, a type of one mixing liquid for cement was investigated to change from the type of two mixing liquids. The α-TCP powder containing 5 wt% calcium chloride powder were mixed with sodium dihydrogen phosphate solution. The HAP content in set cement was approximately 75% at 24 hours after mixing. For estimating in vivo and in vitro reactions, the calcium phosphate cement for rapid crystallization to HAP (HA-CPC) thus prepared was implanted into the abdominal subcutaneous tissues of rats, and immersed into a simulated blood plasma, respectively. The conventional α-TCP cement (TCP-CPC) was also implanted and immersed as a control material. After immersed for 4 weeks, there were no difference between HA-CPC and TCP-CPC. Both calcium phosphate cements had a good biocompatibility. The infiltration of inflammatory cells was confirmed by the tissue of TCP-CPC implantation in rats after 1 and 2 weeks. These cells disappeared at 4 weeks by histopathological examination. In the HA-CPC case, the inflammatory cells were not observed after implanted for 1 week. Moreover, HA-CPC could be strengthened by short fibers of calcium phosphate glass. These results of this study suggest that the clinical application of calcium phosphate cement for rapid crystallization to HAP is possible.
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