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Biochemical analysis of porphyrin compounds in fluorescent tissues of oral cancer

Research Project

Project/Area Number 09672036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

ONIZAKI Kojiro  Institute of Clinical Medicine, University of Tsukuba Assistant Professor, 臨床医学系, 講師 (60194578)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsoral cancer / autofluorescence / porphyrin / adjunct diagnostic method
Research Abstract

This study was carried out to elucidate relationship between autofluorescence emitted from oral cancer and porphyrin compounds. Established cell lines (HSC2,3,4), dimethyl benz [a] anthracen-induced tongue cancer in hamster and clinical samples obtained from patients with oral cancer were biochemically analyzed. Fluorescences emitted from the conditioned medium of cancer cells cultured with 5-aminolevulinic acid were similar to fluorescence spectra of protoporphyrin IX, coproporphyrin and Zn-protoporphyrin IX. Ethyl acetate extracts of nude-mouse tumors formed by HSC2 and HSC3 and of tongue cancer in hamster contained fluorescent compounds like protoporphyrin IX. High performance liquid chromatography of clinical samples revealed that the fluorescent materials were classified into protoporphyrin, coproporphyrin and other types. These results suggested that autofluorescence emitted from oral cancer was associated with porphyrin and cancer cell could accumulate fluorescent compounds such as protoporphyrin IX.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] K.Onizawa: "Usefulness of fluorescence photography for diagnosis of Oral Cancer"Int. J. Oral. Maxillafac. Surg.. 28. 206-210 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Onizawa: "Chromatic analysis of autofluorescence emitted from squamous cell car*as arising in the oral cavity : a preliminary stuydy"Int. J. Oral. Maxillafac. Surg.. 29. 42-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onizawa K, Saginoya H, Furuya Y, Yoshida H, Fukuda H.: "Usefulness of fluorescence photography for diagnosis of oral cancer."Int J Oral Maxillofac Surg. 28. 206-210 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onizawa K, Saginoya H, Yoshida H.: "Chromatic analysis of autofluorescence emitted from squamous cell carcinomas arising in the oral cavity : a preliminary study."Int J Oral Maxillofac Surg. 29. 42-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Cnizawa: "Usefulness of fluorescence photography for diagnosis of oral cancer"Int J Oral Maxillotac Surg. 28. 206-210 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K Onizawa: "Chromatic analysis of autofluorescence emitted from squamous cell carcinomas arising in the oral cavity: aproliminary study"Int J oral Maxillotac surg. 29. 42-46 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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