• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ANALYSIS OF SIGNAL TRANSDUCTION PATHWAY IN BONE INDUCTION BY BONE MORPHOGENETIC PROTEIN

Research Project

Project/Area Number 09672039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTOKYO MEDICAL AND DENTAL UNIVERSITY

Principal Investigator

ASAHINA Izumi  TOKYO MEDICAL AND DENTAL UNIVERSITY,FACULTY OF DENTISTRY,ASSISTANT PROFESSOR, 歯学部, 講師 (30221039)

Co-Investigator(Kenkyū-buntansha) OIDA Shinitiro  FACULTY OF DENTISTRY,INSTRUCTOR, 歯学部, 助手 (10114745)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBMP / Smad / cbfa-1 / signal transduction / 情報伝達 / Cbfa-1
Research Abstract

(1) Phospholilation of mouse Smad-l by BMP
Mouse Smad-1 cDNA fragment of 1.6 kb was obtained from cDNA of mouse bone marrow derived stroma cell : ST-2 using PCR method, and the cDNA in protein expression vector with Flag reporter gene was transfected into ST-2 cell.. The transformed cell was treated with OP-1, BMP-2, and TGF-beta, and then cell lysate was imunoprecipitated with the antibody against Flag protein. As a result, phospholilation of mSmad was observed in the cell treated with these growth factors. These results suggest Smad play important role in the signal transudation pathway of osteoblastic differentiation.
(2) Induction of cbfa-1 gene expression
We examined the gene expression of runt family transcriptional factor, cbfa- 1, which plays important role in bone formation. The treatment of OP-1 to ST-2 or MC3T3-El cell induced induced cbfa-l gene expression two to three times by control after 48 hours. Thus, the result suggests that BMP signal is transmitted to cbfa-l byway of Smad phosphlilation and stimulates osteoblastic differentiation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 小山治彦: "Differential Display 法を用いた Osteogenic Protein-1(OP-1)の骨形成作用に伴う細胞内シグナル伝達に関与する遺伝子の解析" 口腔病学会雑誌. 64. 205-222 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oida,S.et al: "Molecular cloning of DCR amplified BMP-related genes (GDF-5,6 and 7) from rat tooth cells using modified pBluercript Sk+ vector" J.Hard Tiss.Biol. 6. 16-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iimura,T.et al: "Characterization of two length CDNA from human MSX-2 from dental pulp-derived cells" DNA sequence. 8. 87-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 朝比奈 泉: "骨再生の分子機構と骨形成蛋白質(BMP)を用い骨欠損修復への応用" 臨床科学. 34. 1261-1268 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oida,S.et al: "Biological activities of bone extract containing TGFβ super family proteins" J.Hard Tiss.Biol. 7. 27-34 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koyama H: "Analysis of Genes Implicated in Osteogenic Signaling by Osteogenic Protein-1 (OP-1) using Differential" J Stomatological Society. 64. 205-222 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oida, S., Morotome, Y., Nakamura, T.and Terashima, T: "Molecular cloning of PCR amplified BMP-related genes (GDF-5,6 and 7) from rat tooth cells using modified pBluescript SK+ vector." J.Hard Tiss.Biol. 6. 16-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iimura, T., Takeda, K., Goseki, M., Maruoka, Y., Sasaki, S.and Oida, S.: "Characterization of two length cDNA for human Msx-2 from dental pulp-derived cells." DNA Sequence. 8. 87-92 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Asahina I: "Molecular mechanism of bone formation and regenaration of bone defects using bone Morphogenetic protein" Rinsyo kagaku. 34. 1261-1268 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oida, S., Yamazaki, Y., Mori, M., and Asahina, I.: "Biological activities of bone extract containing TGF-beta superfamily proteins ;" J.Hard Tiss.Biol.7. 27-34 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 朝比奈 泉: "骨再生の分子機構と骨形成蛋白質(BMP)を用いた骨欠損修復への応用" 臨床科学. 34・9. 1261-1268 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S. Oida. et. al.: "Biological Activities of Bone Extract cortaing TGF-β Superfamily protein" J. Hand Tiss. Res.7・1. 27-36 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小山治彦: "Differential Display法を用いたOsreogenic Protein-1(OP-1)の骨形成作用に伴う細胞内シグナル伝達に関与する遺伝子の解析" 口腔病学会雑誌. 642. 205-222 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi