Anti-tumor effect and mechanism of the peptide from Lactobacillus plantarum
| Project/Area Number |
09672045
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
AMINO Kayoko Osaka University Faculty of Dentistry Research assistant, 歯学部, 助手 (50202700)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAKUDA Masayoshi Osaka University Faculty of Dentistry Professor, 歯学部, 教授 (00028755)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | Tumor antigen peptide / CTL activity / Muramyl dipeptide (MDP) / 腫瘍抗原ペプチド(PIA) / 癌抗原ペプチド / Muramyldipeptide(MDP) |
|---|
| Research Abstract |
After we transplanted P815 mastocytoma to mice, we inoculated tumor antigen peptide (P1A) and the peptide from Lactobacillus plantarum.We investigated survivalrate of those mice.50% survival rate of those mice that were inocurated tumor antigen peptide (P1A) and the peptide from Lactobacillus plantarum was not elongated.
Then we inoculated tumor antigen peptide (P1A) and Muramyldipeptide (MDP) after we transplanted P815 mastocytoma to mice.And then we inoculated tumor antigen peptide (P1A) and MDP twice weekly 7 days after we transplanted P815 mastocytoma.We took spleen cell from those mice and took up T cell.We investigated cytotoxic T lymphocyte (CTL) activity, IL-2 production, IFN-rproduction of T cell.And we analized CD4 portion and CD8 portion from T cell.We thought that tumor antigen peptide was catched by Antigen presenting cell (APC), helper Tcell was activated and CTL was also activated further more.We could not see CTL activation after we inoculated only tumor antigen peptide.But we could see CTL activation after we inoculated tumor antigen peptide and MDP.
We could not make sure anti-tumor effect of the peptide from Lactobacillus plantarum.
|
Report
(3 results)
Research Products
(13 results)
