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ENHANCING EFFECTS OF EPIDERMAL GROWTHFACTOR ON HUMAN ORAL SQUAMOUS CELL CARCINOMA CELL MOTILITY AND ANALYSIS OF INTERCELLULER SIGNAL TRANSDUCTION

Research Project

Project/Area Number 09672062
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHEALTH SCIENCES UNIVERSITY OF HOKKAIDO

Principal Investigator

SHIBATA Toshiyuki  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO SCHOOL OF DENTISTRY : ASSOCIATED PROFESSOR, 歯学部, 助教授 (50226172)

Co-Investigator(Kenkyū-buntansha) ARISUE Makoto  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO SCHOOL OF DENTISTRY : PROFESSOR, 歯学部, 教授 (20091407)
NAGAYASU Hiroki  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO SCHOOL OF DENTISTRY : INSTRACTOR, 歯学部, 助手 (90265075)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsHUMAN / SCC / ORAL CANCER / EGF / INVASION / MATASTASIS / INVASIN
Research Abstract

1) Effect of EGF in paracrine manner
In order to clear the effects of Epidermal Growth Factor (EGF) on the invasion abilities of human oral squamous cell carcinoma, five cell lines were studied. EGF enhanced the random motility of all cell lines in a dose-dependent fashion and exposure to EGF let to an increased production of urokinase type plasminogen activator and matrix metalloproteniase-9 by the same cells.These results strongly suggest the EGF may promote the invasion and metastasis of human oral squamous cell carcinomas.
2) EFFECT OF EGE IN AUTOCRINE MANNER
Cell lines, which produced EGF, was found out in these cell lines. In these cells, EGF producedby tumor cells can stimulate the random motility itself through the EGF receptor.
3) ESTABLISHMENT OF EGF SENSITIVE CLONE
To reveal the signal pathway, we established s-1 clone cells, which was most sensitive clone against the EGE stimulation and also we established i-1 clone cells, which was most insensitive clone.
4) ANALYSIS OF SIGNAL TRANSDUCTION OF EGF-INDUCED MOTILITY
When EGE bind to the EGF receptor, tyrosine phosphorelation is firstly occurred and subsequently PKC is activated through the activation of PLCr arising from erbB homodimer and br activation of P13-kinase arising from erbB3 heterodimer.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Toshiyuki SHIBATA: "Enhancement of Tumor Antigen Expression and Inhibition of Pulmonary Metastasis of Rat Fibrosarcoma Cells by Local Radiotherapy." Anticancer Research. 17. 317-322 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshiyuki SHIBATA: "Enhancement of Tumor Associated Antigen Expression During the Regression Phase of Xenogenized Tumor Cell Growth in vivo." Anticancer Research. 17. 2135-2140 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takashi KAWANO: "Enhancement by Epidermal Growth Factor(EGF)of Motility of Human Oral Squamous cell Carcinoma Cells in an Autocrine Manner" Jpn.J.Maxillofac.Surg.44. 16-21 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroki NAGAYASU: "Reversible and Irreversible Tumor Progression of a Weekly Malignant Rat Mammary Carcinoma Cell Line by In Vitro Exposure to Epidermal Growth Factor" International Journal of Oncology. 12. 197-202 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Futoshi OKADA: "Inflamatory Cell-Mediated Tumor Progression and Minisatellite Mutation Correlate with the Decrease of Antioxidative Enzymes in Murine Fibrosarcoma Cells" British Journal of Cancer. 79. 377-385 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takashi KAWANO: "Inhibition of Enhanced Motility Stimulated by EGF in Subclone(s-1)Cells of Human Oral Squamous Cell Carcinoma Cell Line(Ca9-22)" Jpn.J.Maxillofac.Surg.44. 725-731 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.NAGAYASU,J-I.HAMADA,T.KAWANO,S.KONAKA,D.NAKATA,T.SHIBATA,M.ARISUE,M.HOSOKAWA,N.TAKEICHI,T.MORIUCHI: "INHIBITORY EFFECTS OF MALOTILATE ON INVASION AND METASTASIS OF RAT MAMMARY CARCINOMA CELLS BY MODIFYING THE FUNCTIONS OF VASCULAR ENDOTHERIAL CELLS" BRITISH JOURNAL OF CANCER. 77(9). 1371-1377 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.NAGAYASU,J-I.HANADA,D.NAKATA,T.SHIBATA,M.KOBAYASHI,M.HOSOKAWA,N.TAKEICHI: "REVERSIBLE AND IRREVERSIBLE TUMOR PROGRESSION OF A WEAKLY MALIGNANT RAT MAMMARY CARCINOMA CELL LINE BY IN VITRO EXPOSURE TO EPIDERMAL GROWTH FACTOR" INTERNATIONAL JOURNAL OF ONCOLOGY. 12. 197-202 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.SHIBATA,K.SUZUKI,T.YAMASHITA,N.TAKEICHI,M.MICALLEF,M.HOSOKAWA,H.KOBAYASHI,M.ARISUE: "IMMUNOLOGICAL ANALYSIS OF ENHANCED SPONTANEOUS METASTASIS IN WKA RATS FOLLOWING CRYOSURGE Y" ANTICANCER RES.18. 2483-2486 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I.CHIBA,M.MUTHUMALA,Y.YAMAZAKI,A.ZAMAN,T.IIZUKA,A.AMEMIA,T.SHIBATA,H.KASHIWAZAKI,C.SUGIURA,H.FUKUDA: "CHARACTERISTICS OF MUTAIONS IN THE P53 GENE OF ORAL SQUAMOUS CELL CARCIMONAS ASSOCIATED WITH BETEL CHEWING IN SRILANKA." INTERNATIONAL JOURNAL OF CANCER. 77. 839-842 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.SHIBATA,T.YAMASHITA,K.SUZUKI,N.TAKEICHI,M.MICALLEF,M.HOSOKAWA,H.KOBAYASHI,M.MURATA,M.ARISUE.: "ENHANCEMENT OF EXPERIMENTAL PULMONARY METASTASIS AND INHIBITION OF SUBCUTANEOUSLY TRANSPLANTED TUMOR GROWTH FOLLOWING CRYOSURGERY" ANTICANCER RES.18. 4443-4448 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] F.OKADA,K.NAKAI,T.KOBAYASHI,T.SHIBATA,S.TAGAMI,Y.KAWAKAMI,T.KITAZAWA,R.KOMIMAMI,S.YOSHIMURA,K.SUZUKI,N.TANIGUCHI,O.INANAMI,M.KUWABARA,H.KISHIDA,D.NAKAE,Y.KONISHI,T.MORIUCHI,M.HOSOKAWA: "INFLAMMATORY-CELL-CELLMEDIATED TUMOR PROGRESION AND MINISATELLITE MUTATION CORRELATE WITH THE DECREASE OF ANTIOXIDATIVE ENZYMES IN MURINE FIBROSARCOMA CELLS." BRITISH JOURNAL OF CANCER. 79. 377-385 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.KAWANO,T.SHIBATA,H.NAGAYASU,H.KITAJO,D.NAKATA,M.ARISUE: "ENHANCEMENT BY EPIDERMAL GROWTH FACTOR(EGF)OF MOTILITY OF HUMAN ORAL SQUAMOUS CELL CARCINOMA CELLS IN AN AUTOCRINE MANNER" JPN.J.ORAL MAXILOFAC.SURG. 44. 16-21 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.KAWANO,T.SHIBATA,H.NAGAYASU,H.KITAJO,D.NAKATA,K.NAKAI,M..ARISUE: "INHIBITION OF ENHANCED MOTILITY STIMULATED BY EGF IN SUBCLONE(S-1)CELLS OF HUMAN ORAL SQUAMOUS CELL CARCINOMA CELL LINE(CA9-22)-----EFFECTS OF INHIBITION OF INTERCELLULAR SIGNAL TRANSDUCTION ON CELL MOTILITY" JPN.J.ORAL MAXILOFAC.SURG. 44. 725-731 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshiyuki SHIBATA: "Enhancement of Tumor Antigen Expression and Inhibition of Pulmonary Metastasis of Rat Fibrosarcoma Cells by Local Radiotherapy." Anticancer Research. 17. 317-322 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshiyuki SHIBATA: "Enhancement of Tumor Associated Antigen Expression During the Regression Phase of Xenogenized Tumor Cell Growth in vivo." Anticancer Research. 17. 2135-2140 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takashi KAWANO: "Enhancement by Epidermal Growth Factor(EGF) of Motility of Human Oral Squamous cell Carcinoma Cells in an Autocrine Manner" Jpn.J.Maxillofac.Surg.44. 16-21 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hiroki NAGAYASU: "Reversible and Irreversible Tumor Progression of a Weekly Malignant Rat Mammary Carcinoma Cell Line by In Vitro Exposure to Epidermal Growth Factor" International Journal of Oncology. 12. 197-202 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Futoshi OKADA: "Inflamatory Cell-Mediated Tumor Progression and Minisatellite Mutation Correlate with the Decrease of Antioxidative Enzymes in Murine Fibrosarcoma Cells" British Journal of Cancer. 79. 377-385 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takashi KAWANO: "Inhibition of Enhanced Motility Stimulated by EGF in Subclone(s-1) Cells of Human Oral Squamous Cell Carcinoma Cell Line (Ca9-22)" Jpn.J.Maxillofac.Surg.44. 725-731 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.SHIBATA: "Enhancement of Tumor Antigen Expression and Inhibition of Pulmonary Metastasis of Rat Fibrosarcoma Cells by Local Radiotherapy" ANTICANCER RESEARCH. 17. 317-322 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.SHIBATA: "The LEC Rat as an Animal Model for Bilirubin-Induced Tooth Pigmentation" ANTICANCER RESEARCH. 17. 2141-2146 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.SHIBATA: "Enhancement of Tumor Associated Antigen Expression During the Regression Phase of Xenogenized Tumor Cell Growth in vivo" ANTICANCER RESEARCH. 17. 2135-2140 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 河野 峰: "EGFオートクリン作用によるヒト口腔扁平上皮癌細胞の運動能促進" 日本口腔外科学会雑誌. 44. 16-21 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.NAGAYASU: "Reversible and Irreversible Tumor Progression of a Weakly Malignant Rat Mammary Carcinoma Cell Line by in vitro Exposure to Epidermal Growth Factor" INTERNATIONAL JOURNAL OF ONCOLOGY. 12. 197-202 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.NAGAYASU: "Inhibitory Efects of Malotilate on Invasion and Metastasis of Rat Mammary Carcinoma Cells by Modifying the Functions of Vascular Endithelial Cells" British Journal of Cancer. (in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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