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The mechanisms involved in the estrogen action on the development and proliferation of oral disease

Research Project

Project/Area Number 09672073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

OZONO Satoru  Kanagawa Dental College, School of Dentistry assistant professor, 歯学部, 講師 (40084785)

Co-Investigator(Kenkyū-buntansha) ONOZUKA Minoru  Gifu University, School of Medicine assistant professor, 医学部, 講師 (90084780)
ITO Yumi  Kanagawa Dental College, School of Dentistry research associate, 歯学部, 助手 (00176372)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsestrogen receptor / ameloblastoma / adenoid cystic carcinoma / human / DMBA / submandibular gland tumor / rat / in situ hybridization法 / 口腔病変 / プロゲステロン受容体
Research Abstract

Immunohistochemical analysis of estrogen receptor was carried out in salivary adenoid cystic carcinoma and ameloblastoma. In addition, we have tested the presence or absence the antitumor activity of the antiestrogen, tamoxifen, using the DMBA rat submandibular gland tumor model.
In these specimens, in agreement with the results seen with the breast cancer tissue used as positive control, strongly-stained cells, in which the ER-immunoreactivity was located in the cell nucleus, were detected. Accordingly, the cases were classed as ER-positive or ER-negative by scoring them on the basis of the visually estimated percentage of tumor cells showing positive nuclear staining, followed by an evaluation using a 10% cut-off level. In human tumors, ER immunoreactivity was positive in 5 (41.7% of total cases ; 3 female and 2 male) of 12 cases of salivary ACC, 11(50% of total cases ; 6 female and 5 male) of 22 cases of ameloblastoma, and one case (female) ot malignant ameloblastoma. The metastatic tumors, which had obtained from the patients with ER positive tumors, showed also immunoreactivity in a large proportion of the tumor cells.
In separate experiments, the antitumor effects of tamoxifen, an antiestrogen drug, on DMBA-induced ER-positive submandibular gland tumor in rats were studied. Submandibular gland tumors appeared from about 3 months after transplantation of DMBA pellet to female rats, and more than half of them were ER positive. Tamoxifen inhibited the growth of DMBA-induced ER-positive submandibular gland tumors in rats. Thus, this anti-tumor agent might have clinical potential for use against ER-positive salivary adenoid cystic carcinomas.
These results, showing ER expression in human salivary adenoid cystic carcinomas and malignant ameloblastoma, suggest that the presence of this receptor is one of the most useful tools for predicting the response to endocrine therapy for these tumors with poor prognosis

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 小園 知: "エナメル上皮腫における性ホルモン・レセプターの発現について" 日本病理学会誌. 86・1. 266 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小園 知: "ラット歯胚形成期におけるエストロゲン受容体の組織化学的検索" 歯基礎誌. 40. 172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito, Y: "The expression of estrogen and progesterone receptor in sialoadenitis." J.Oral Pathol.Med.27(7). 357 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横矢重俊: "肉芽腫性口唇炎5例の臨床病理学的検討" 日口科誌. 47・1. 56-60 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita, Y: "Periodontal ligament cell culture on the hydrophobic substance coated with proteins of periodontal ligament fibroblast-conditioned medium" J.Biomater.Sci.Polymer Edn.9・5. 489-505 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto, T.: "Origin of primary sensory neurons innervating the buccal stretch receptor" J Dent Res. 78・1. 49-53 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ozono, S., Ito, Y., Kubota, N.and Sato, K.: "Expression of sex-hormone receptor in ameloblastoma." Proc Jpn Soc Pathol. 86(1). 266 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto, T., Hirayama, A.and Ozono, S.: "An HRP study of the distribution of primary afferent neurons innervating the buccal stretch receptor in rats." Bull Tokyo dent Coll. 38. 307-310 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita, Y., Hidaka, T., Ozono, S.and Kawase, T.: "Periodontal ligament cell culture on teh hydrophobic substance coated with proteins of periodontal ligament fibroblast-conditioned medium." J Biomater Sci Polymer Edn. 9(5). 489-505 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue, S., Kinoshita, Y., Honma, Y., Mizunuma, H.and Ozono, S.: "A case of secondary syphilis with eruption of oral mucosa." J Jpn Oral Muco Membr. 4. 69-73 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Onozuka, M., Watanabe, K., Takada, H., Mirbod, SM., Karasawa, N., Nagasaki, I., Nishiyama, K.and Ozono, S.: "Glial reaction linked with p70-induced seizure activity in the cerebral cortex of rats." Neurosci res com. 23(2). 111-117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito, Y., Sato, K.and Ozono, S.: "The expression of estrogen and progesterone receptor in sialoadenitis." J.Oral Pathol.Med.27(7). 357 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto, T., Onozuka, M., Nagasaki, S., Watanabe, K.and Ozono, S.: "Origin of primary sensory neurons innervating the buccal stretch receptor." J Dent Res. 78(1). 49-53 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小園 知: "エナメル上皮腫における性ホルモン・レセプターの発現について" 日本病理学会誌. 86・1. 266 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Yamamoto: "An HRP study of the distribution of primary affernet neurons innervating the buccal stretch receptor in rats" Bull.Tokyo Dent.Coll.38・4. 307-310 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小園 知: "ラット歯胚形成期におけるエストロゲン受容体の免疫組織化学的検索" 歯基礎誌. 40. 172 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横矢重俊: "肉芽腫性口唇炎5例の臨床病理学的検討" 日口科誌. 47・1. 56-60 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y,Kinoshita: "Periodontal ligament cell culture on the hydrophobic substance coated with proteins of periodontal ligament fibroblast-conditioned medium" J.Blomater.Sci.Polymer Edn.9・5. 489-505 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 井上 聡: "口腔粘膜疹を主症状とした第2期梅毒の1例" 日口粘膜誌. 4・1. 69-73 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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