| Project/Area Number |
09672074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
HONMA Yoshiro Kanagawa Dental College, Dental school, lecture, 歯学部, 講師 (60157112)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNUMA Hideyuki Kanagawa Dental College, Dental school, assistant, 歯学部, 助手 (70267516)
KAWAHARA Kenji Kanagawa Dental College, Dental school, assistant, 歯学部, 助手 (80247311)
INOUE Satoshi Kanagawa Dental College, Dental school, assistant, 歯学部, 助手 (50139557)
OZONO Satoru Kanagawa Dental College, Dental school, lecture, 歯学部, 講師 (40084785)
KINOSHITA Yukihiko Kanagawa Dental College, Dental school, associate Professer, 歯学部, 助教授 (70084770)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | oral carcinoma / precancerous lesion / Human Papilloma Virus / proto-oncogene / multi-primary carcinoma / precancerous condition |
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| Research Abstract |
1. Multi-primary oral squamous cell carcinoma was encountered predominantly in women, and it was commonly localized in the buccal mucosa and the gingiva. The subsequent appearance of a third group of the lesions commonly represented on early cancer which tented to develop after a shorter time interval.
2. As a background factor, the presence of precancerous lesion was predominant as observed in 7 patients (luekoplakia in 6 and erythroplakia in 1), and 3 patients had a precancerous condition (lichen planus in 2 and, plummer-Vinson syndrome in 1). Linkage to alcohol use or smoking habits was not clear.
3. Histopathologically, epithlial hyperplasia and severe dysplasia were observed in the epithelium of precancerous lesions and in the adjacent epithelium of carcinoma. Immunohistochemical investigation showed HPV16 frequ-ently, and HPV16 was localized commonly in the upper layers to the spinous cells.
4. Electron microscopy revealed 50- to 60-nm viral particles in the epithelial nuclei in precancerous lesions and in the adjacent epithlium of carcinoma. The viral particles were identified to be HPV16 by immuno-electron microscopy.
5. PCR assay revealed that HPVI6 DNA was detected in 7out of 9 patients in the tumor.
These results suggest that HPV16 infected oral mucosa has a potential for multi-occurence of malignant tumor igenesis.
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