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APPLICATION OF DNA POLYMORPHISMS TO PERSONAL IDENTIFICATION IN FORENSIC ODONTOLOGY

Research Project

Project/Area Number 09672120
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

MINAGUCHI Kiyoshi  TOKYO DENTAL COLLEGE,DEPARTMENT OF DENTISTRY,PROFESSOR, 歯学部, 教授 (00133380)

Co-Investigator(Kenkyū-buntansha) HAGA Toshiaki  TOKYO DENTAL COLLEGE,DEPARTMENT OF DENTISTRY,ASSIATANT, 歯学部, 助手 (80287185)
HANAOKA Yoichi  TOKYO DENTAL COLLEGE,DEPARTMENT OF DENTISTRY,LECTURER, 歯学部, 講師 (30180912)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsPersonai identification using DNA analysis / D12S67 / D12S66 / D21S11 / VWA / DYS19 / Amelogenin / Material examination / D12S67の塩基配列多型 / 非ヒト霊長類のD12S67 / 非ヒト霊長類のD21S11 / LPL / 非ヒト霊長類のVWA / 歯からのDNA多型 / 法医学的物体検査 / D12S67. / amelogenin / 性別判定 / 義歯からのDNA多型 / 熱処理歯牙からのDNA多型
Research Abstract

The purpose of this study is preparation of database on several DNA polymorphisms and their application to personal identification in the field of forensic dentistry and in the case of mass disaster. Differential PCR amplification of D12S67 locus resulted in increase in the number of discrete alleles from 11 to 35 and increase of heterozygosity from 80% to 93%. Comparison of nucleotide sequences of 188 alleles further increased the number of alleles to 52 and heterozygosity to 96%. Polymorphisms of D12S66, D21S11, VWA, DYS 19, and D4S43 loci were investigated using 170, 141, 155, 119 and 131 samples, respectively. The heterozygosity calculated for D12S66, D21S1, VWA and D4S43 were 57, 80, 79 and 81%, respectively.
The frequency of allele 15 was dominated in the Japanese population in DYS 19. New alleles with different nucleotide sequences from the known alleles were found in D21S11 and VWA loci, and the conformation of the nucleotide sequence of allele 14 was different from other alleles in the VWA locus. Allele 1 of the D4S43 locus was further classified into 4 alleles, resulting in the increase of heterozygosity of the locus. Above mentioned loci could successfully applied to the detection of polymorphisms using teeth as a source of DNA.
Comparison of the nucleotide sequences of D12S67, D21S11 and VWA loci in primates supported the known divergence models of primates and also showed some characteristics of typical mutation pattern of STR.Unknown regions of the X and Y amelogeniri genes were sequenced and new methods of sex determination using highly degraded DNA was established. Amplification of DNA was possible from teeth heat-treated at 200゚C.Detection of DNA from saliva-stained resin pieces was also possible from samples left at room temperature until 2 months. We also showed theoretically that three polymorphisms, D12S67, D21SI11 and VWA, could discriminate individuals in a mass disaster with victims of more than 1000.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Kiyoshi Minaguchi: "Comparison of the nucleotide sequences and partial amplification of the D12S67 locus" ADVANCES IN RESEARCH ON DNA POLYMORPHISMS. (TOYOSHOTEN). 76-83 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masatsugu Inoue et al.: "DNA extraction from saliva-stained resin pieces as dental material and amplification to personal identification" ADVANCES IN RESEARCH ON DNA POLYMORPHISMS. (TOYOSHOTEN). 261-266 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水口清 他: "D12S67 locusの塩基配列の多型" DNA多型. Vol.5. 154-158 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 井上雅嗣 他: "歯科材料(レジン)に付着した唾液斑からのDNA分析" DNA多型. Vol.5. 74-78 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "D12S67 locus, a bipartite lucus, Differential amplification of parts of the nucleotide sequence reveals considerable polymorphism" Electrophoresis. 18. 1923-1927 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoichi Hanaoka et al.: "D4S43 locus DNA typing in the Japanese population and applicarion to teeth with degraded DNA" J Forenc Sci. 43(2). 406-409 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Sekiguchi et al.: "PCR detection of the human amelogenin gene and its application to the diagnosis of amelogenesis imperfecta" Bull Tokyo dent Coll. 39. 275-285 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水口清 他: "霊長類のD12S67 locusおよびD21S11 locus" DNA多型. Vol.6. 110-115 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水口清: "歯科とDNA検査-法歯学の立場から-1. いま、なぜDNAなのか?" ザ・クイントエッセンス. Vol.16(10). 33-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水口清: "歯科とDNA検査-法歯学の立場から-2. DNAの変異と進化および個人識別" ザ・クイントエッセンス. Vol.16(11). 28-31 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 水口清: "歯科とDNA検査-法歯学の立場から-3. 口腔領域に由来する資料からのDN検査" ザ・クイントエッセンス. Vol.16(12). 25-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "The DYS19 system in the Japanese population and its detection using teeth as a source of DNA" Bull Tokyo dent Coll. 40. 21-26 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "Comparison of the nucleotide sequences and partial amplification of the D12S67 locus" ADVANCES IN RESEARCH ON DNA POLYMORPHISMS,TOYOSHOTEN. 76-83 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masatsugu Inoue et al.: "DNA extraction of saliva-stained resin pieces as dental material and amplification to personal identification" ADVANCES IN RESEARCH ON DNA POLYMORPHISMS,TOYOSHOTEN. 261-266 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi et al.: "Nucleotide sequence polymorphism of D12S67 locus" DNA POLYMORPHISM. Vol.5. 154-158 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masatsugu Inoue et al.: "DNA analysis on saliva-stained dental materials (dental resin)" DNA POLYMORPHISM. Vol.5. 74-78 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "D12S67 locus, a bipartite locus ; Differential amplification of parts of the nucleotide sequence reveal considerable polymorphism." Electrophoresis.18. 1923-1927 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "Dentistry and DNA analysis in dentistry-From standpoint of Forensic Odontology-Why DNA attracts the public eye?" The Quintessence.Vol.16(10). 33-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "Dentistry and DNA analysis in dentistry-From standpoint of Forensic Odontology-Mutation of DNA,evolution and personal identification" The Quintessence. Vol.16(11). 28-31 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "Dentistry and DNA analysis in dentistry-From standpoint of Forensic Odontology-DNA analysis using materials derived from oral region" The Quintessence.Vol.16(12). 25-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Sekiguchi et al.: "PCR detection of the human amelogenin gene and its application to the diagnosis of amelogenesis imperfecta" Bull Tokyo dent Coll. 39. 275-285 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoichi Hanaoka et al.: "D4S43 locus DNA typing in the Japanese population and application to teeth with degraded DNA" J Forens Sci. 43. 406-409 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi et al.: "D12S67 and D21S11 locus in primates" DNA POLYMORPHISM. Vol.6. 110-115 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kiyoshi Minaguchi: "The DYS19 system in the Japanese population and its detection using teeth as a source of DNA" Bull Tokyo dent Coll. 40. 21-26 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoichi Hanaoka et al.: "D4S43 locus DNA typing in the Japanese population and applicarion to teeth with degraded DNA" J Forenc Sci. 43(2). 406-409 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyoshi Minaguchi: "The DYS19 system in the Japanese population and its detection using teeth as a source of DNA." Bull Tokyo dent Coll. 40(1). in press (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 水口 清他: "霊長類のD12S67 locusおよびD21S11 locus" DNA多型. Vol.6. 110-115 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 水口 清: "歯科とDNA検査-法医学の立場から-1.いま、なぜDNAなのか?" ザ・クイントエッセンス. Vol.16(10). 33-36 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 水口 清: "歯科とDNA検査-法医学の立場から-2.DNAの変異と進化および個人識別" ザ・クイントエッセンス. Vol.16(11). 28-31 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 水口 清: "歯科とDNA検査-法医学の立場から-3.口腔領域に由来する資料からのDN検査" ザ・クイントエッセンス. Vol.16(12). 25-28 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyoshi Minaguchi: "Comparison of the nucleotide sequences and partial amplification of the D12S67 locus" ADVANCES IN RESEARCH ON DNA POLYMORPHISMS(TOYOSHOTEN). 76-83 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masatsugu Inoue et al.: "DNA extraction from saliva-stained resin pieces as dental material and amplification to personal identification" ADVANCES IN RESEARCH ON DNA POLYMORPHISMS(TOYOSHOTEN). 261-266 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 水口 清,他: "D12S67 locusの塩基配列の多型" DNA多型. Vol.5. 154-158 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 井上雅嗣,他: "歯科材料(レジン)に付着した唾液斑からのDNA分析" DNA多型. Vol.5. 74-78 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiyoshi Minaguchi: "D12S67 locus,a bipartite locus,Differential amplification of the nucleotide sequence reveals considerable polymorphism" Electrophoresis. 18. 1923-1927 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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