| Project/Area Number |
09672227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
IMAGAWA Masayoshi Grad. Sch. of Pharm. Sci., Osaka University Assoc. Prof., 薬学研究科, 助教授 (20136823)
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| Co-Investigator(Kenkyū-buntansha) |
今川 正良 大阪大学, 薬学研究科, 助教授 (20136823)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | transcription / gene expression / silencer / DNA-binding protein |
|---|
| Research Abstract |
The expression of glutathione transferase-P (GST-P) gene is rarely detected in normal liver, whereas it is highly induced during hepatocarcinogenesis of rat. We identified the silencer region in the GST-P promoter, and isolated the silencer binding proteins. In this project, we characterized the silencer binding proteins and mapped the functional (repressive) domains.
1) We cloned nuclear factor-1 (NF1) as a silencer binding protein, and NF1-A had a repressive activity. The minimal repression domain was also identified.
2) We cloned the genomic clone of NF1-A and identified the all exon-intron boundaries.
3) The splicing isoforms of NF1-B was isolated, and these proteins were found to function as both positive and negative transcription factors.
4) We characterized the DNA-binding specificities and affinities of NF1-A, -B, -C and -X.
5) The nuclear localization signals in the NF1-A were identified.
6) C/EBPδgene is regulated through C/EBP binding sites at the downstream region by autoregulation mechanism.
7) The novel silencer binding proteins were isolated by yeast one-hybrid system
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